Mouse Anti-TSA Recombinant Antibody (clone D2.3) (CAT#: PABZ-125)
Recombinant Mouse Antibody (D2. 3) is capable of binding to TSA, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry
Figure 1 Hydrolysis of NP-ester 2p catalysed by antibodies D2.3 and D2.4.
(a) The time-course of p-nitrophenol (4p) release following the addition of NP-ester 2p (0.25 mM) to antibody D2.3 (0.35 μM) or D2.4 (0.1 μM). Inset, Rate of hydrolysis (v = dP/st) as a function of time (derived from equation (1); see Materials and Methods); the initial and steady-state rates od D2.3 (vᵢ and vf, respectively) are noted. (b) The steady-state rate, vf, and the lag rate constant, klag, exhibited by antibody D2.3 (1.5 μM) at different concentrations of ester 2p. The steady-state rate (vf) and concentration of substrate 2p (S₀) were fitted to the Michaelis-Menten model giving a KM and vfmax values of 25 μM and 7.8 μM min⁻¹, respectively.
Lindner, A. B., Eshhar, Z., & Tawfik, D. S. (1999). Conformational changes affect binding and catalysis by ester-hydrolysing antibodies1. Journal of molecular biology, 285(1), 421-430.
Figure 2 Binding affinities of ligands and their effect on pre-equilibrium of antibody D2.3.
Lindner, A. B., Eshhar, Z., & Tawfik, D. S. (1999). Conformational changes affect binding and catalysis by ester-hydrolysing antibodies1. Journal of molecular biology, 285(1), 421-430.
Specifications
- Immunogen
- N-{[2-({[1-(4carboxybutanoyl)amino]-2-phenylethyl}-hydroxyphosphinyl)oxy]acetyl}-2-phenylethyl-amine
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- TSA
- Clone
- D2.3
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody D2.3 has been reported in application of ELISA. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1009 | Recombinant Anti-TSA Antibody | WB, Dot, FuncS | IgG |
| MHH-1009 | Recombinant Human Anti-TSA Antibody | WB, FC, FuncS | IgG |
| PABL-351 | Mouse Anti-TSA Recombinant Antibody (clone 1D4) | IB, IP | Mouse IgG |
| PABL-352 | Mouse Anti-TSA Recombinant Antibody (clone 6D9) | WB, ELISA, FuncS | Mouse IgG |
| PABW-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.4) | ELISA | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1009-F(E) | Recombinant Anti-TSA Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-1009-F(E) | Recombinant Human Anti-TSA Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
| PFBL-351 | Mouse Anti-TSA Recombinant Antibody (clone 1D4); Fab Fragment | WB, ELISA, IF, FuncS | Mouse Fab |
| PFBL-352 | Mouse Anti-TSA Recombinant Antibody (clone 6D9); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| PFBZ-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.3); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1009-S(P) | Recombinant Anti-TSA Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-1009-S(P) | Recombinant Human Anti-TSA Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| PSBZ-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.3); scFv Fragment | ELISA | Mouse scFv |
| PSBW-125 | Mouse Anti-TSA Recombinant Antibody (clone D2.4); scFv Fragment | ELISA | Mouse scFv |
| PSBC-126 | Mouse Anti-TSA Recombinant Antibody (clone D2.5); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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