Mouse Anti-ACACA Recombinant Antibody (VS3-CJ103) (CAT#: VS3-CJ103)
This product is a mouse antibody that recognizes ACACA.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36650/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center, cytosol & actin filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36650/if_selected.jpg
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows cytoplasmic positivity in adipocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_B_1_4_selected.jpg
(Immunohistochemical staining of human breast shows cytoplasmic positivity in adipocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_B_1_4_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_B_8_5.jpg
(Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_8_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_8_5.jpg
Figure 7 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_6_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/63018/141436_A_7_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000278540-ACACA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000278540-ACACA
Specifications
- Immunogen
- A Synthetic peptide of human ACACA (N-terminus)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ACACA
- Species Reactivity
- Human
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- ACACD; ACACalpha; ACC; ACC1; ACCA; ACCalpha; Acac1; hACC1; ACACA
- Gene ID
- 31
- UniProt ID
- Q13085
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Phosphorylation at Ser-80 by AMPK inactivates enzyme activity.
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
- Protein Refseq
- NP_942131.1; NP_942133.1; NP_942134.1
- Function
- Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed:20952656, PubMed:20457939, PubMed:29899443).
This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:20952656, PubMed:20457939, PubMed:29899443).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ACACA"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4059z | Mouse Anti-ACACA Recombinant Antibody (clone 37G1) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
| VS3-WK797 | Rabbit Anti-ACACA Recombinant Antibody (clone ST53-08) | WB, IHC | Rabbit IgG |
| VS3-XY15 | Mouse Anti-ACACA Recombinant Antibody (clone 2G3) | WB, IP, IF | Mouse IgG2b |
| VS3-FY10 | Recombinant Rabbit Anti-ACACA Antibody (clone R05-3D6) | WB | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0027 | Hi-Affi™ Rabbit Anti-ACACA Recombinant Antibody (clone DS27AB) | WB, IHC, ICC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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