Mouse Anti-ADA Recombinant Antibody (VS3-CJ221) (CAT#: VS3-CJ221)
This product is a mouse antibody that recognizes ADA (C-term).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/26_D7_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/26_D7_2_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/27_D7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/27_D7_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/28_D7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/28_D7_1_blue_red_green.jpg
Figure 4 Duodenum
(Enterocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes - Microvilli
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/162544_A_7_2.jpg
(Enterocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes - Microvilli
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/162544_A_7_2.jpg
Figure 5 Testis
(Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/162544_A_5_6.jpg
(Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1399/162544_A_5_6.jpg
Figure 6 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196839-ADA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196839-ADA
Figure 6 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196839-ADA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196839-ADA
Specifications
- Immunogen
- KLH-conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human ADA
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A/G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:4000).
Target
- Alternative Names
- ADA1
- Gene ID
- 100
- UniProt ID
- P00813
- Sequence Similarities
- Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.
- Cellular Localization
- Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Lysosome, Membrane
- Protein Refseq
- NP_000013.2
- Function
- Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed:8452534, PubMed:16670267).
Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed:20959412).
Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed:11772392).
Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity).
Enhances CD4+ T-cell differentiation and proliferation (PubMed:20959412).
Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed:23193172).
Stimulates plasminogen activation (PubMed:15016824).
Plays a role in male fertility (PubMed:21919946, PubMed:26166670).
Plays a protective role in early postimplantation embryonic development (By similarity).
Recommended Products
Recommended Dilution Buffer
Related Resources
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ADA"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1230z | Mouse Anti-ADA Recombinant Antibody (clone 20C10) | FC, IHC, WB | Mouse IgG2b |
| MRO-215-MZ | Mouse Anti-ADA Recombinant Antibody (clone ADA-1) | RIA, WB, IP | Mouse IgG |
| MOB-0430MZ | Recombinant Mouse Anti-Escherichia coli ada Antibody (clone BEB-2) | IP, RIA, WB | Mouse antibody |
| VS3-QX22 | Mouse Anti-ADA Recombinant Antibody (clone 608CT2.1.3) | WB | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0067 | Hi-Affi™ Rabbit Anti-ADA Recombinant Antibody (clone DS67AB) | ICC, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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