Rabbit Anti-ANXA6 Recombinant Antibody (clone R07-7G6) (CAT#: VS3-FY1650)
Clone R07-7G6 is a rabbit IgG recombinant monoclonal antibody that has been developed to detect human ANXA6 protein, a calculated weight of 76 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R07-7G6 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:50-1:100), immunoprecipitation (dilution range 1:20).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9977/1914_F7_14_cr5c87753c9ceb1_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9977/1914_F7_14_cr5c87753c9ceb1_selected.jpg
Figure 2 Smooth muscle
(High expression in smooth muscle)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2462/9636_B_4_7_val_selected.jpg
(High expression in smooth muscle)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2462/9636_B_4_7_val_selected.jpg
Figure 3 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5077/15117_A_1_2_selected.jpg
(Immunohistochemical staining of human appendix shows cytoplasmic positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5077/15117_A_1_2_selected.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_8_5.jpg
Figure 5 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_6_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_6_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_8.jpg
Figure 7 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_3.jpg
Figure 8 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9650/24355_A_7_4.jpg
Figure 9 RNA cell line category: Cell line enhanced (ASC diff)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197043-ANXA6
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000197043-ANXA6
Specifications
- Immunogen
- Recombinant human Annexin-6 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat ANXA6
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-7G6
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 76 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Kwashiorkor and Papillary Hidradenoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Annexin A6; 7 KDa Calelectrin; Chromobindin-20; Lipocortin VI; Annexin-6; CPB-II; ANX6; P68; P70; Testis Secretory Sperm-Binding Protein Li 198a
- Gene ID
- 309
- UniProt ID
- P08133
- Sequence Similarities
- Belongs to the annexin family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated in response to growth factor stimulation.
- Protein Refseq
- NP_001146.2; NP_001180473.1; NP_001350043.1
- Function
- May associate with CD21. May regulate the release of Ca²⁺ from intracellular stores.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ANXA6"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2911z | Mouse Anti-ANXA6 Recombinant Antibody (clone 35H1) | WB, ELISA | Mouse IgG1 |
| ZG-0290F | Mouse Anti-ANXA6 Recombinant Antibody (ZG-0290F) | WB | Mouse IgG |
| VS3-QX55 | Mouse Anti-ANXA6 Recombinant Antibody (clone 8A7-F8-G12) | WB | Mouse IgG1 |
| VS4-WK33 | Mouse Anti-ANXA6 Recombinant Antibody (VS4-WK33) | ELISA, WB | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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