Rabbit Anti-AURKB Recombinant Antibody (clone 4E5) (CAT#: ZG-0563J)

Figure 1 Rabbit Anti-AURKB Antibody (ZG-0563J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
All lanes: AURKB antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 36, 17, 35 kDa
Observed band size: 40 kDa

Figure 2 Rabbit Anti-AURKB Antibody (ZG-0563J) in IHC

IHC image of ZG-0563J diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-AURKB Antibody (ZG-0563J) in IHC

IHC image of ZG-0563J diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 4 Rabbit Anti-AURKB Antibody (ZG-0563J) in IP

Immunoprecipitating AURKB in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0563J in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0563J(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)