Rabbit Anti-BAG2 Recombinant Antibody (clone R04-4A5) (CAT#: VS3-XY3273)
The antibody is a recombinant rabbit antibody against BAG cochaperone 2 (BAG2), which acts as a nucleotide-exchange factor (NEF) promoting the release of ADP. It specifically reacts with human, mouse and rat BAG2. It has been reported for use in Western Blot, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18862/175_C9_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18862/175_C9_1_selected.jpg
Figure 2 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_4_6.jpg
Figure 3 Smooth muscle
(Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_B_5_7.jpg
(Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_B_5_7.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19918/47056_A_9_8.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112208-BAG2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112208-BAG2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BAG2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-4A5
- Applications
- WB, ICC, IF
- Related Disease
- Chronic Frontal Sinusitis; Cystic Fibrosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
Target
- Alternative Names
- BAG-2; dJ417I1.2
- Gene ID
- 9532
- UniProt ID
- O95816
- Long Name
- BAG cochaperone 2
- Cellular Localization
- Axon, Cytoxol
- Post Translation Modifications
- Ubiquitination at Lys5, Lys123, Lys154, and Lys200 (NX_O95816 [NX_O95816-1])
Modification sites at PhosphoSitePlus (O95816)
Glycosylation from GlyGen (O95816) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011513301.1; XP_005249547.1
- Function
- Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BAG2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0888MZ | Mouse Anti-BAG2 Recombinant Antibody (clone CBLS-163) | WB | Mouse IgG1 |
| VS3-FY1604 | Rabbit Anti-BAG2 Recombinant Antibody (clone R09-7C3) | WB, IP | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0319 | Hi-Affi™ Rabbit Anti-BAG2 Recombinant Antibody (clone DS319AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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