Rabbit Anti-BLNK Recombinant Antibody (clone R04-7H1) (CAT#: VS3-FY1666)
The R04-7H1 recombinant monoclonal antibody is designed to specifically target human BLNK protein, a calculated weight of 50 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R04-7H1 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/1779_G1_1_selected.jpg
(Immunofluorescent staining of human cell line REH shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/1779_G1_1_selected.jpg
Figure 2 Tonsil
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_4_8_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_4_8_val_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/102126_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/102126_B_7_5.jpg
Figure 4 Colon
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Gradient Gradient: Intensity: Not representative Quantity: None Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_9_3.jpg
(Endocrine cells Staining: Medium Intensity: Moderate Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Gradient Gradient: Intensity: Not representative Quantity: None Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_9_3.jpg
Figure 5 Kidney
(Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_7_5.jpg
(Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_7_5.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/101333_A_7_8.jpg
Figure 7 Spleen
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/102126_B_7_4.jpg
(Cells in red pulp Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/38309/102126_B_7_4.jpg
Figure 8 RNA cell line category: Group enriched (Daudi, REH, RPMI-8226, U-698)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095585-BLNK
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000095585-BLNK
Specifications
- Immunogen
- Synthetic peptide of human BLNK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BLNK
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-7H1
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 50 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Hypoglobulinemia and absent B cells
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- B Cell Linker; B-Cell Adapter Containing A SH2 Domain Protein; Src Homology [SH2] Domain-Containing Leukocyte Protein Of 65 KD; Src Homology 2 Domain-Containing Leukocyte Protein Of 65 KDa; B-Cell Adapter Containing A Src Homology 2 Domain Protein; B Cell Adaptor Containing SH2 Domain; Cytoplasmic Adapter Protein; B-Cell Activation; SLP-65
- Gene ID
- 29760
- UniProt ID
- Q8WV28
- Cellular Localization
- Cytoplasm, Cell membrane
- Post Translation Modifications
- Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN.
Modification sites at PhosphoSitePlus (Q8WV28)
Modification sites at neXtProt (NX_Q8WV28)
- Protein Refseq
- NP_001107566.1; NP_001245369.1; NP_001245370.1; NP_001245371.1; NP_037446.1
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BLNK"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1548z | Mouse Anti-BLNK Recombinant Antibody (clone 30G10) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| VS3-QX121 | Mouse Anti-BLNK Recombinant Antibody (clone 520CT6.1.1) | WB | Mouse IgM |
| VS3-WK125 | Mouse Anti-BLNK Recombinant Antibody (clone G1-F9) | WB, ELISA | Mouse IgG1 |
| VS3-XY126 | Mouse Anti-BLNK Recombinant Antibody (clone 5G9) | ELISA, WB, IHC | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0363 | Hi-Affi™ Rabbit Anti-BLNK Recombinant Antibody (clone DS363AB) | ELISA, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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