Rabbit Anti-BRD4 Recombinant Antibody (clone 1E5) (CAT#: ZG-0573J)

Figure 1 Rabbit Anti-BRD4 Antibody (ZG-0573J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: BRD4 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 153, 81, 89 kDa
Observed band size: 153 kDa

Figure 2 Rabbit Anti-BRD4 Antibody (ZG-0573J) in IHC

IHC image of ZG-0573J diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-BRD4 Antibody (ZG-0573J) in IHC

IHC image of ZG-0573J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.