This product is a recombinant Llama antibody that can recognize C3. hC3Nb1 against human C3b and show that it binds with lownanomolar or picomolar affinity to native C3, C3b, iC3b, and the C3(H2O) mimic C3-methylamine(C3MA) and yields efficient inhibition of the
alternative pathway in human and murine serum. hC3Nb1 is shown to prevent both proconvertase assembly as well as binding of the C3 substrate to C3 convertases. hC3Nb1 is an efficient inhibitor of FH cofactor activity.
Figure 1 The hC3Nb1 sdAb inhibits C3 cleavage at the substrate level.
Assay in 1 % human serum on a mannan-coated LP activating surface. The horizontal axis gives the concentration of sdAbs added to the serum. The vertical axis shows the level of C3 fragment deposition with 100 % deposition defined as the signal from serum without added sdAb. The dotted line indicates the final molar concentration of C3 in the assay based on a concentration of 5.3 μM in undiluted plasma.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Figure 2 The hC3Nb1 is a potent inhibitor of the alternative pathway.
Assay in mouse serum on a zymosan-coated alternative pathway activating surface depicted as in panel A. The dotted line represents the final molar concentration of C3 in the assay assuming 4.2 μM C3 in 100 % serum from C57BL6 male mice.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Figure 3 hC3Nb1 inhibits convertase assembly and FH mediated FI cleavage of C3b due to spatial overlap with FH and FB.
SEC analysis of the AP proconvertase with and without hC3Nb1. The Nb inhibits the formation of the AP proconvertase only when the N122 linked glycan is present on FB.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Figure 4 SPR analysis of the interaction of hC3Nb1 with C3b and C3.
Representative SPR concentration series for C3 are displayed. The measured (gray) and fitted (red) SPR curves are shown for 50, 20, 10, 5, 2, 1, 0.25 and 0.1 nM for C3b or 20, 10, 5, 2.5, 1.25, 0.61, 0.32 and 0.15 nM for C3.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
Figure 5 The hC3Nb1 sdAb also inhibits C3 cleavage at the substrate level.
Assay in 1 % human serum on a mannan-coated LP activating surface. The horizontal axis gives the concentration of sdAbs added to the serum. The vertical axis shows the level of C3 fragment deposition with 100 % deposition defined as the signal from serum without added sdAb.
Jensen, R. K., Pihl, R., Gadeberg, T. A., Jensen, J. K., Andersen, K. R., Thiel, S., ... & Andersen, G. R. (2018). A potent complement factor C3–specific sdAb inhibiting multiple functions in the alternative pathway of human and murine complement. Journal of Biological Chemistry, 293(17), 6269-6281.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-142-S(P) | Recombinant Anti-human C3 Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
MHH-142-S(P) | Recombinant Human Anti-human C3 Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
HPAB-0182-YC-S(P) | Human Anti-C3 Recombinant Antibody; scFv Fragment (HPAB-0182-YC-S(P)) | Neut | Humanized scFv |
HPAB-M0014-YC-S(P) | Mouse Anti-C3 Recombinant Antibody (clone cC3-4); scFv Fragment | ELISA, Neut | Mouse scFv |
HPAB-M0015-YC-S(P) | Human Anti-C3 Recombinant Antibody (clone hC34); scFv Fragment | ELISA, Neut | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-080CQ | Mouse Anti-C3 Recombinant Antibody (clone K13/16) | ELISA, IP, WB, Neut | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-081CQ | Recombinant Mouse Anti-C3 Antibody (3E7) | ELISA, FC, BL, IF, RIA | IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0414 | Hi-Affi™ Rabbit Anti-C3 Recombinant Antibody (clone DS414AB) | IHC-P, IHC-Fr | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0182-YC-F(E) | Human Anti-C3 Recombinant Antibody; Fab Fragment (HPAB-0182-YC-F(E)) | Neut | Humanized Fab |
HPAB-0062-FY-F(E) | Human Anti-C3 Recombinant Antibody (clone MOR09611); Fab Fragment | ELISA | Human Fab |
HPAB-0064-FY-F(E) | Human Anti-C3 Recombinant Antibody (clone MOR9674); Fab Fragment | ELISA | Human Fab |
FAMAB-0160JF-F(E) | Rabbit Anti-C3 Recombinant Antibody (clone BA8); Fab Fragment | IHC, ELISA, FuncS | Rabbit Fab |
FAMAB-0161JF-F(E) | Rabbit Anti-C3 Recombinant Antibody (clone MA11); Fab Fragment | IHC, ELISA, FuncS | Rabbit Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-167CQ | Rat Anti-C3 Recombinant Antibody (clone CBLS-197) | WB, FC, IA, IF, IHC, IHC-Fr, IHC-P, IP | Rat IgG2a |
HPAB-0939LY | Human Anti-C3 Recombinant Antibody (clone SS3-4) | ELISA | Humanized IgG1 |
MOR-0028-FY | Mouse Anti-C3 Recombinant Antibody (clone 5G9) | FuncS | Mouse IgG2a |
MOR-0029-FY | Mouse Anti-C3 Recombinant Antibody (clone 3E7) | FC, FuncS, IF | Mouse IgG1 |
ZG-0302U | Rabbit Anti-C3 Recombinant Antibody (clone E28-P) | IHC-P | Rabbit IgG |
There are currently no Customer reviews or questions for PABC-558. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.