Mouse Anti-Cadherin Recombinant Antibody (clone CH-19) (CAT#: VS-0224-XY18)
The recombinant antibody recognizes to pan cadherin and can be used as a marker for the plasma membrane in cells which express cadherins. It can be used for Western blot, Flow cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry-paraffin and Immunohistochemistry-frozen.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/1030_A11_3_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/1030_A11_3_selected.jpg
Figure 2 Small intestine
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Paneth cells
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_6_2.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Paneth cells
Staining: High
Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_6_2.jpg
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/23616/52785_A_9_3.jpg
Figure 4 RNA cell line category: Cell line enhanced (CACO-2, OE19, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079112-CDH17
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000079112-CDH17
Specifications
- Immunogen
- The synthetic peptide, DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponds to amino acids 889-912 of Chicken N-Cadherin (cdh2).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Cadherin
- Species Reactivity
- Human, Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Cow, Cat, Canine, Pig, Xenopus, Snake, Zebrafish
- Clone
- CH-19
- Applications
- Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry-frozen, Immunohistochemistry-paraffin
- Related Disease
- Diffuse Gastric; Lobular Breast Cancer Syndrome
Product Property
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested in Western blot (1/1000-1/2000), Flow cytometry (1-2 µl per million cells), Immunofluorescence (1/10-1/200), and Immunohistochemistry (1/10-1/200).
Target
- Alternative Names
- Cadherin; CDH; Calcium-dependent adhesion
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-146 | Recombinant Anti-cadherin Antibody | WB, Dot, FuncS | IgG |
| MHH-146 | Recombinant Human Anti-cadherin Antibody | ELISA, WB, FC, FuncS | IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-146-F(E) | Recombinant Anti-cadherin Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| MHH-146-F(E) | Recombinant Human Anti-cadherin Antibody Fab Fragment | IF, FC, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-146-S(P) | Recombinant Anti-cadherin Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| MHH-146-S(P) | Recombinant Human Anti-cadherin Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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