Rabbit Anti-CALU Recombinant Antibody (clone R05-1E7) (CAT#: VS3-FY1981)
Clone R05-1E7 is a rabbit recombinant monoclonal antibody that attaches to human CALU. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. This calumenin antibody was used in western blot analysis of calumenin in Hela, A549, C6 lysates and immunohistochemistry analysis of paraffin-embedded human colon cancer.
Specific Inquiry
WB
Figure 1 Anti-CALU Antibody (VS3-FY1981) in WB.
The Calumenin expression in Hela, A549, C6 lysates were detected using the anti-Calumenin by western blot assay.
IHC
Figure 2 Anti-CALU Antibody (VS3-FY1981) in IHC.
Immunohistochemistry staining of paraffin-embedded human colon cancer using the anti-Calumenin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Specifications
- Immunogen
- Synthetic peptide of human Calumenin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CALU
- Species Reactivity
- Human, Rat
- Clone
- R05-1E7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 37 kDa, while the observed molecular weight stands at 47 kDa.
- Related Disease
- Human colon cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Calumenin; IEF SSP 9302; Multiple EF-Hand Protein; Crocalbin-Like Protein; Crocalbin
- Gene ID
- 813
- UniProt ID
- O43852
- Sequence Similarities
- Belongs to the CREC family.
- Cellular Localization
- Endoplasmic reticulum, Golgi apparatus, Membrane, Sarcoplasmic reticulum, Secreted
- Post Translation Modifications
- Glycosylation at Thr60, Ser261, and Asn131 (NX_O43852 [NX_O43852-1])
Ubiquitination at Lys84, Lys94, Lys98, Lys255, and Lys114 (NX_O43852 [NX_O43852-1])
Modification sites at PhosphoSitePlus (O43852)
Glycosylation from GlyConnect CALU_HUMAN (1062)
Glycosylation from GlyGen (O43852) 9 sites, 45 N-linked glycans (1 site), 3 O-linked glycans (8 sites)
- Protein Refseq
- NP_001124146.1; NP_001186600.1; NP_001186601.1; NP_001186602.1; NP_001210.1
- Function
- Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CALU"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3224z | Mouse Anti-CALU Recombinant Antibody (clone 39B12) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| ZG-0360F | Mouse Anti-CALU Recombinant Antibody (ZG-0360F) | WB | Mouse IgG |
| VS4-WK55 | Mouse Anti-CALU Recombinant Antibody (VS4-WK55) | ELISA, WB | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0444 | Hi-Affi™ Rabbit Anti-CALU Recombinant Antibody (clone DS444AB) | WB, IHC, ICC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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