Rabbit Anti-CASP8 Recombinant Antibody (clone R02-0D7) (CAT#: VS3-FY1673)

• Immunogen
• Synthetic peptide of human Caspase-8

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Hamster CASP8

• Species Reactivity
• Human, Hamster

• Clone
• R02-0D7

• Applications
• Western Blot

• MW
• The calculated weight of the target protein is approximately 55 kDa, while the observed molecular weight stands at 55 kDa.

• Related Disease
• Huntington disease

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000

• Alternative Names
• Caspase 8; Caspase 8, Apoptosis-Related Cysteine Peptidase; Caspase 8, Apoptosis-Related Cysteine Protease; MORT1-Associated Ced-3 Homolog; ICE-Like Apoptotic Protease 5; Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; FADD-Like ICE; Casp-8; FLICE; CAP4

• Gene ID
• 841

• UniProt ID
• Q14790

• Sequence Similarities
• Belongs to the peptidase C14A family.

• Cellular Localization
• Cell projection, Cytoplasm, Nucleus

• Post Translation Modifications
• Generation of the p10 and p18 subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
  Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis (PubMed:20937773).
  Phosphorylation on Tyr-380 by SRC is mediated by interaction with the SRC SH2 domain and does not affect dimerization or recruitment to the death-inducing signaling complex (DISC) but negatively regulates DISC-mediated processing and activation of CASP8, down-regulating its proapoptotic function (PubMed:16619028, PubMed:27109099).
  Phosphorylation on Tyr-380 also enhances localization to lamellipodia in migrating cells (PubMed:18216014).

• Protein Refseq
• XP_005246950.1; XP_011510271.1; XP_047301915.1; XP_047301916.1; XP_047301917.1

• Function
• Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood.
  Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death.
  Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10.
  Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody