Mouse Anti-CCN1 Recombinant Antibody (clone 093G9) (CAT#: PABC-443)
This product is a recombinant Mouse antibody that can recognize CCN1. 093G9, is effective to antagonize the effects of CCN1 and hence has potential therapeutic benefits against rheumatoid arthritis. The epitope recognized by 093G9 is mapped to residues 77 to 80 of CCN1, and a peptide encompassing residues 75 to 81 of CCN1 displays high binding affinity for 093G9.
Specific Inquiry
Inhib
Figure 1 Anti-Cyr61 mAb 093G9 inhibits proliferation of FLS and IL-6 production by FLS of RA patients in vitro.
(A) The proliferation of FLS was inhibited by anti-Cyr61 Ab 093G9 compare with control IgG. (B) Cyr61-stimulated production of IL-6 by FLS was inhibited by 093G9.
Lin, J., Zhou, Z., Huo, R., Xiao, L., Ouyang, G., Wang, L., ... & Li, N. (2012). Cyr61 induces IL-6 production by fibroblast-like synoviocytes promoting Th17 differentiation in rheumatoid arthritis. The Journal of Immunology, 188(11), 5776-5784.
Inhib
Figure 2 CCN1-stimulated production of IL-1β by HaCaT was inhibited by 093G9.
HaCaT cells were pretreated with 20μg/ml of an anti-CCN1 monoclonal antibody (named 093G9, black bar) or control IgG (open bar) for one hour.
Sun, Y., Zhang, J., Zhai, T., Li, H., Li, H., Huo, R., ... & Teng, J. (2017). CCN1 promotes IL-1β production in keratinocytes by activating p38 MAPK signaling in psoriasis. Scientific reports, 7, 43310.
FC
Figure 3 CD4+ and CD130+ cells in splenocytes and lymph node cells from 093G9-treated or control mice analyzed using flow cytometry.
Lin, J., Zhou, Z., Huo, R., Xiao, L., Ouyang, G., Wang, L., ... & Li, N. (2012). Cyr61 induces IL-6 production by fibroblast-like synoviocytes promoting Th17 differentiation in rheumatoid arthritis. The Journal of Immunology, 188(11), 5776-5784.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human CCN1
- Species Reactivity
- Human
- Clone
- 093G9
- Applications
- Inhib, FC, ELISA, Neut
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Inhibition, Flow Cytometry, Enzyme-linked Immunosorbent Assay and Neutralization. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-443 | Mouse Anti-CCN1 Recombinant Antibody (clone 093G9); Fab Fragment | ELISA, Neut | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-443 | Mouse Anti-CCN1 Recombinant Antibody (clone 093G9); scFv Fragment | ELISA, Neut | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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