Rabbit Anti-CCNE1 Recombinant Antibody (clone 3A5) (CAT#: ZG-0019U)

The purified Rabbit Anti-CCNE1 Recombinant Antibody (clone 3A5) displayed high specificity against the CCNE1 protein of human.


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  • MSDS
  • COA
IHC

Figure 1 Rabbit Anti-CCNE1 Antibody (ZG-0019U) in IHC

Figure 1 Rabbit Anti-CCNE1 Antibody (ZG-0019U) in IHC

IHC image of ZG-0019U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IF

Figure 2 Rabbit Anti-CCNE1 Antibody (ZG-0019U) in IF

Figure 2 Rabbit Anti-CCNE1 Antibody (ZG-0019U) in IF

Immunofluorescence staining of Hela Cells with ZG-0019U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).


Specifications

  • Immunogen
  • A synthesized peptide derived from human Cyclin E1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human CCNE1
  • Species Reactivity
  • Human
  • Clone
  • 3A5
  • Applications
  • ELISA, IHC, IF
  • Conjugate
  • Unconjugated
  • Related Disease
  • Clear Cell Adenocarcinoma Of The Ovary and Retinoblastoma

Product Property

  • Purification
  • Affinity purified
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
    IHC:1:50-1:200, IF:1:20-1:200

Target

  • Alternative Names
  • G1/S-specific cyclin-E1; CCNE1; CCNE

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CCNE1"

Intrabody

CAT Product Name Application Type
IAB-B078(A) Recombinant Anti-human CCNE1 Intrabody [(D-Arg)9] FACS, ICC, FuncS scFv-(D-Arg)9
IAB-B078(G) Recombinant Anti-human CCNE1 Intrabody [+36 GFP] PCA, WB, FuncS scFv-Tat
IAB-B078(T) Recombinant Anti-human CCNE1 Intrabody [Tat] FACS, ELISA, IF, FuncS scFv-Tat

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0517 Hi-Affi™ Rabbit Anti-CCNE1 Recombinant Antibody (clone DS517AB) FC, IF, IP, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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