This product is a recombinant human antibody that recognizes CD133. The antibody was purified by affinity chromatography. It can be used in WB, ELISA, FC.
Figure 1 Immunoblotting analysis confirms the specificity of CD133-scFv-1.
The proteins of PLC/PRF/5 cells (transfected with CD133 shRNA or control shRNA) were subjected to immunoblot analysis using anti-CD133-scFv-1 (1:50) or anti-β-actin (1:5000).
Figure 2 Flow cytometry analysis confirms the specificity of CD133-scFv-1.
To determine changes in CD133-scFv-1 cell binding affinity, transfected PLC/PRF/5 cells were stained with anti-CD133-scFv-1/PE and cells were sorted using a flow cytometer. Ten thousand cells were sorted per experiment (n = 3).
Figure 3 Competitive ELISA of murine monoclonal antibody and purified human scFv (Binding at OD450).
Control 1: 50 μl 293C3 and 50 μl 3% BSA-PBS (uninhibited control); Control 2: only antigen and secondary antibody (HRP-goat-anti-mouse antibody).
Figure 4 Competitive ELISA of purified human scFv and murine monoclonal antibody: Binding of CD133-scFv-1 binding at OD450.
(A) Control 1: 50 μl murine monoclonal antibody (0.833 μg/mL) and 50 μl 3% BSA-PBS (uninhibited control); Control 2: only antigen and secondary antibody (HRP-protein A).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
ZG-032R | Mouse Anti-CD133 Recombinant Antibody (ZG-032R) | WB | Mouse IgG |
ZG-033R | Mouse Anti-CD133 Recombinant Antibody (ZG-033R) | WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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