Mouse Anti-CD147 Recombinant Antibody (clone OX114) (CAT#: FAMAB-1634CQ)

This product is a recombinant mouse antibody that recognizes CD147. The antibody was purified by affinity chromatography. It can be used in IP, WB, FC, IHC.


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FC

Figure 1 OX114 mAb binds both two and three domain forms of mouse CD147.

Figure 1 OX114 mAb binds both two and three domain forms of mouse CD147.

Flow cytometry of OX114 mAb binding transfected 293T cells. Light grey line represents untransfected cells, black line represents cells expressing mouse CD147 d1+2, dark grey line represents cells expressing mouse CD147d0-2.

Hanna, S. M., Kirk, P., Holt, O. J., Puklavec, M. J., Brown, M. H., & Barclay, A. N. (2003). A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically. BMC biochemistry, 4(1), 1-9.

WB

Figure 2 Western blot of transfected 293T lysates.

Figure 2 Western blot of transfected 293T lysates.

Lane U, untransfected cells, 2d, cells transfected with mouse CD147 d1+2, 3d, cells transfected with mouse CD147 d0-2. Each lane contains lysate from 106 cells.

Hanna, S. M., Kirk, P., Holt, O. J., Puklavec, M. J., Brown, M. H., & Barclay, A. N. (2003). A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically. BMC biochemistry, 4(1), 1-9.

Figure 3 Ability of OX114 mAb to bind to recombinant protein on the Biacore.

Figure 3 Ability of OX114 mAb to bind to recombinant protein on the Biacore.

Hanna, S. M., Kirk, P., Holt, O. J., Puklavec, M. J., Brown, M. H., & Barclay, A. N. (2003). A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically. BMC biochemistry, 4(1), 1-9.


Specifications

  • Immunogen
  • Recombinant mCD147d0-2rCD4d3+4
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • The 2- and 3-domain isoforms of CD147
  • Species Reactivity
  • Mouse
  • Clone
  • OX114
  • Applications
  • IP, WB, FC, IHC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Gavilimomab; 244096-20-6; ABX-CBL; ABX-CBL; BSG; basigin (Ok blood group); basigin (OK blood group), OK; basigin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone OX114"

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Rabbit Monoclonal Antibody

Recombinant Antibody

CAT Product Name Application Type
FAMAB-1636CQ Mouse Anti-CD147 Recombinant Antibody (clone OX47) RIA, WB, FC, IHC-Fr Mouse IgG1

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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