Mouse Anti-CD200RLc Recombinant Antibody (clone OX132) (CAT#: FAMAB-1614CQ)

Figure 1 Specificity of OX110, OX131 and OX132 mAb.

Streptavidin coupled magnetic beads were coated with biotinylated chimeric proteins containing extracellular domains of members of the mouse CD200R family and rCD4d3+4 and a biotinylation site (as indicated on top of each column). The binding of the mAb indicated in each row was analyzed by flow cytometry. (A) Protein coating levels for each group of magnetic beads were tested by staining with OX68 (CD4 mAb) (tinted solid line) or OX21 (control mAb) (thin line). Flow cytometry plot named rCD4 d3+4 indicates coating level for biotinylated rCD4d3+4 only. (B-D) OX110 (B), OX131 (C) and OX132 (D) mAb were used to stain magnetic beads coated with the chimeric proteins indicated above each column (tinted solid line), or control beads coated with biotinylated rCD4d3+4 only (dashed line). Data are representative of three experiments.

Akkaya, M., Aknin, M. L., Akkaya, B., & Barclay, A. N. (2013). Dissection of agonistic and blocking effects of CD200 receptor antibodies. PLoS One, 8(5), e63325.