Rabbit Anti-CD3D Recombinant Antibody (VS3-CJ339) (CAT#: VS3-CJ339)
This product is a rabbit antibody that recognizes CD3D.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line JURKAT shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71778/1970_F1_3_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71778/1970_F1_3_selected.jpg
Figure 2 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167286-CD3D
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167286-CD3D
Figure 2 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167286-CD3D
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000167286-CD3D
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD3D
- Species Reactivity
- Human
- Applications
- WB, ICC, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- T3D; IMD19; CD3DELTA; CD3-DELTA; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD_antigen: CD3d
- Gene ID
- 915
- UniProt ID
- P04234
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
- Protein Refseq
- NP_000723.1; NP_001035741.1
- Function
- Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD3D"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1284z | Mouse Anti-CD3D Recombinant Antibody (clone 21C10) | WB, ELISA | Mouse IgG1 |
| VS3-WK1275 | Mouse Anti-CD3D Recombinant Antibody (clone AI504783) | ELISA, WB, IHC | Mouse IgG2a |
| VS3-FY282 | Recombinant Mouse Anti-CD3D Antibody (clone 6B6B12) | ELISA, FC, ICC | Mouse IgG2b |
| VS3-FY283 | Recombinant Mouse Anti-CD3D Antibody (clone 1H12G5) | ELISA | Mouse IgG1 |
| VS3-FY284 | Recombinant Mouse Anti-CD3D Antibody (clone 4B9G9) | ELISA, WB, FC | Mouse IgG2b |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-159 | Anti-Human CD3 Recombinant Antibody (Teplizumab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0557 | Hi-Affi™ Rabbit Anti-CD3D Recombinant Antibody (clone DS557AB) | WB, IHC, ICC, IP | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-594-FY-S(P) | Human Anti-CD3D Recombinant Antibody (clone 8E3); scFv Fragment | ELISA | Human scFv |
| HPAB-0703-FY-F(E) | Mouse Anti-CD3D Recombinant Antibody (clone AB002); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0722-FY-F(E) | Mouse Anti-CD3D Recombinant Antibody (clone AB039); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0381-FY-S(P) | Human Anti-CD3D Recombinant Antibody; scFv Fragment (HPAB-0381-FY-S(P)) | FC, ELISA | Human scFv |
| HPAB-0382-FY-S(P) | Human Anti-CD3D Recombinant Antibody; scFv Fragment (HPAB-0382-FY-S(P)) | Activ | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-159 | Afuco™ Anti-CD3D ADCC Recombinant Antibody (Teplizumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0703-FY-S(P) | Mouse Anti-CD3D Recombinant Antibody (clone AB002); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0381-FY-F(E) | Human Anti-CD3D Recombinant Antibody; Fab Fragment (HPAB-0381-FY-F(E)) | FC, ELISA | Human Fab |
| HPAB-0382-FY-F(E) | Human Anti-CD3D Recombinant Antibody; Fab Fragment (HPAB-0382-FY-F(E)) | Activ | Human Fab |
| HPAB-0384-FY-F(E) | Rat Anti-CD3D Recombinant Antibody; Fab Fragment (HPAB-0384-FY-F(E)) | ELISA | Rat Fab |
| HPAB-1490-FY-S(P) | Human Anti-CD3D Recombinant Antibody (clone G19-4); Fab Fragment | WB, ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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