Rabbit Anti-CD53 Recombinant Antibody (clone R01-8U5) (CAT#: VS3-XY3300)
It is a recombinant rabbit antibody targeting the CD53 (CD53 molecule), which involves in growth regulation in hematopoietic cells. It recognizes and binds to human CD53. It has been determined using Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/50-1/100 and 1/50-1/200, respectively.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_B_7_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_B_7_5.jpg
Figure 2 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_7_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_7_4.jpg
Figure 3 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_8_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_8_5.jpg
Figure 4 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_9_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/47216/108620_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, HMC-1, Karpas-707, U-266/70, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143119-CD53
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143119-CD53
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, HMC-1, Karpas-707, U-266/70, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143119-CD53
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143119-CD53
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CD53
- Species Reactivity
- Human
- Clone
- R01-8U5
- Applications
- WB, IHC-P, ICC, IF, FC
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- MOX44; TSPAN25
- Gene ID
- 963
- UniProt ID
- P19397
- Long Name
- CD53 molecule
- Cellular Localization
- Cell junction, Cell membrane, Membrane
- Post Translation Modifications
- Glycosylation at Asn129 and Asn148 (NX_P19397 [NX_P19397-1])
Modification sites at PhosphoSitePlus (P19397)
Glycosylation from GlyGen (P19397) 2 sites
- Protein Refseq
- XP_047290969.1; XP_047290970.1; XP_047290971.1; XP_054195697.1; XP_054195698.1
- Function
- Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD53"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0569 | Hi-Affi™ Rabbit Anti-CD53 Recombinant Antibody (clone DS569AB) | WB, IHC, ICC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ746 | Mouse Anti-CD53 Recombinant Antibody (clone C53-2) | IF, FC | Mouse IgG2a |
| VS3-XY366 | Mouse Anti-CD53 Recombinant Antibody, FITC (clone 4AC53-2) | FC, IF | Mouse IgG2a |
| VS3-XY367 | Mouse Anti-CD53 Recombinant Antibody, PE (clone 4AC53-2) | FC, IF | Mouse IgG2a |
| VS3-XY368 | Mouse Anti-CD53 Recombinant Antibody (clone 4AC53-2) | FC, IF | Mouse IgG2a |
| VS3-WK1483 | Mouse Anti-CD53 Recombinant Antibody (clone COC53) | FC, IF | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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