Rabbit Anti-CD55 Recombinant Antibody (clone 1A5) (CAT#: ZG-0592J)

This product is a rabbit antibody that recognizes CD55.


Specific Inquiry
  • Size:
  • Conjugation:
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  • Purity:
  • Fc Engineering:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-CD55 Antibody (ZG-0592J) in WB

Figure 1 Rabbit Anti-CD55 Antibody (ZG-0592J) in WB

Western Blot
Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate
All lanes: CD55 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42, 49, 40, 57, 60 kDa
Observed band size: 70 kDa

IHC

Figure 2 Rabbit Anti-CD55 Antibody (ZG-0592J) in IHC

Figure 2 Rabbit Anti-CD55 Antibody (ZG-0592J) in IHC

IHC image of ZG-0592J diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC

Figure 3 Rabbit Anti-CD55 Antibody (ZG-0592J) in IHC

Figure 3 Rabbit Anti-CD55 Antibody (ZG-0592J) in IHC

IHC image of ZG-0592J diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human CD55
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human CD55
  • Species Reactivity
  • Human
  • Clone
  • 1A5
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Uconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • CR; TC; DAF; CROM; CHAPLE; complement decay-accelerating factor; CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Cromer blood group antigen; Rh blood group D antigen

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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