Human Anti-CD69 Recombinant Antibody (clone HAIM29) (CAT#: FAMAB-0103-CN)
This product is a human antibody that recognizes CD69. The antibody HAIM29 can be used in some immunoassay techniques such as: ELISA, FC.
Specific Inquiry
FC
Figure 1 Flow cytometric analysis of hAIM-29 mAb.
Results of FACS analysis demonstrated that hAIM-29 can bind to RBL-CD69 but not RBL-WT cells,indicating the specificity of hAIM-29 against human CD69.
Molina, A., Valladares, M., Magadán, S., Sancho, D., Viedma, F., Sanjuan, I., ... & González-Fernández, Á. (2003). The use of transgenic mice for the production of a human monoclonal antibody specific for human CD69 antigen. Journal of immunological methods, 282(1-2), 147-158.
FC
Figure 2 Two-color staining with Abs using RBL-CD69 as target cells.
Two-color staining on RBL-CD69 cells using the commercial mouse PE-conjugated anti-CD69 (TP1/55) mAb and the HuIgM antibodies hAIM-29 (left) and D15-17 (right) followed by FITC-conjugated secondary antibodies.
Molina, A., Valladares, M., Magadán, S., Sancho, D., Viedma, F., Sanjuan, I., ... & González-Fernández, Á. (2003). The use of transgenic mice for the production of a human monoclonal antibody specific for human CD69 antigen. Journal of immunological methods, 282(1-2), 147-158.
WB
Figure 3 hAIM-29 mAb recognizes human CD69 by immunoblot.
Direct immunoblot analysis of total cell lysates (a) and after antigen immunoprecipitation with Protein A-mouse TP1/55 mAb (b), from RBL-CD69 and RBL-WT cells, subjected to SDS-PAGE and transferred to a membrane for the incubation with the hAIM-29 mAb.
Molina, A., Valladares, M., Magadán, S., Sancho, D., Viedma, F., Sanjuan, I., ... & González-Fernández, Á. (2003). The use of transgenic mice for the production of a human monoclonal antibody specific for human CD69 antigen. Journal of immunological methods, 282(1-2), 147-158.
ELISA
Figure 4 hAIM-29 mAb recognizes human CD69 in ELISA
A comparison between anti-CD69 murine mAbs (TP1/8, TP1/33, TP1/55) and the human hAIM-29 for the specific recognition of human CD69 Ag, analyzed by ELISA using CD69-Fc bound to a 96-well polystyrene plate.
Molina, A., Valladares, M., Magadán, S., Sancho, D., Viedma, F., Sanjuan, I., ... & González-Fernández, Á. (2003). The use of transgenic mice for the production of a human monoclonal antibody specific for human CD69 antigen. Journal of immunological methods, 282(1-2), 147-158.
Specifications
- Immunogen
- Human CD69-transfected rat cells
- Host Species
- Human
- Type
- Human IgM
- Specificity
- Human CD69
- Species Reactivity
- Human
- Clone
- HAIM29
- Applications
- ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone HAIM29"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1417z | Mouse Anti-CD69 Recombinant Antibody (clone 40B11) | IHC, FC, FuncS | Mouse IgG1 |
| MOB-0359MZ | Recombinant Mouse Anti-Human CD69 Molecule Antibody (clone GN51) | ELISA | Mouse antibody |
| ZG-0901F | Mouse Anti-CD69 Recombinant Antibody (clone SMM) | FC | Mouse IgG1 |
| VS3-QX198 | Mouse Anti-CD69 Recombinant Antibody (clone 8B6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-WK45 | Mouse Anti-CD69 Recombinant Antibody (clone E6-B6) | WB, ELISA | Mouse IgG1 |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-330LC-F(E) | Human Anti-CD69 Recombinant Antibody; Fab Fragment (TAB-330LC-F(E)) | FC, ELISA | Human Fab |
| TAB-331LC-F(E) | Human Anti-CD69 Recombinant Antibody; Fab Fragment (TAB-331LC-F(E)) | FC, ELISA | Human Fab |
| TAB-332LC-F(E) | Human Anti-CD69 Recombinant Antibody; Fab Fragment (TAB-332LC-F(E)) | FC, ELISA | Human Fab |
| TAB-333LC-F(E) | Human Anti-CD69 Recombinant Antibody; Fab Fragment (TAB-333LC-F(E)) | FC, ELISA | Human Fab |
| TAB-334LC-F(E) | Human Anti-CD69 Recombinant Antibody; Fab Fragment (TAB-334LC-F(E)) | FC, ELISA | Human Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4069 | Hi-Affi™ Rabbit Anti-CD69 Recombinant Antibody (clone SI136DS) | ELISA, WB, IP | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0103-CN-S(P) | Human Anti-CD69 Recombinant Antibody (clone HAIM29); scFv Fragment | ELISA, FC | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0103-CN-F(E) | Human Anti-CD69 Recombinant Antibody (clone HAIM29); Fab Fragment | ELISA, FC | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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