Mouse Anti-CD74 Recombinant Antibody (VS3-CJ338) (CAT#: VS3-CJ338)

• Immunogen
• KLH-conjugated synthetic peptide encompassing a sequence within human CD74

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human CD74

• Species Reactivity
• Human

• Applications
• IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.2% BSA, PBS.

• Preservative
• 0.01% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunohistochemistry (1:100-1:300).

• Alternative Names
• II; p33; CLIP; DHLAG; HLADG; Ia-GAMMA

• Gene ID
• 972

• UniProt ID
• P04233

• Cellular Localization
• Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane

• Post Translation Modifications
• N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

• Protein Refseq
• NP_001020329.1; NP_001020330.1; NP_004346.1

• Function
• Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  Class-II-associated invariant chain peptide
  Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.
  Isoform p41:
  Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215).
  Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS