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  • Rabbit Anti-CD82 Recombinant Antibody (clone R06-7K5)

Rabbit Anti-CD82 Recombinant Antibody (clone R06-7K5) (CAT#: VS3-XY3301)

The product is a recombinant rabbit antibody against CD82 (CD82 molecule), which associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. It specifically reacts with human and rat CD82. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin and Flow cytometry.


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Specific Inquiry
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  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1143_C8_4_selected.jpg
Figure 2 IHC staining of human appendix
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_2_2_selected.jpg
Figure 3 IHC staining of human tonsil
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows distinct positivity of reticular fibers.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2508/ihc_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1130_C8_1_blue_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61418/1242_A11_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_B_9_5.jpg
Figure 7 Pancreas
Figure 7 Pancreas
(Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_1_3.jpg
Figure 8 Kidney
Figure 8 Kidney
(Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_9_5.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28900/64792_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
Figure 10 RNA cell line category: Cell line enhanced (HMC-1, U-87 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000085117-CD82

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat CD82
  • Species Reactivity
  • Human, Rat
  • Clone
  • R06-7K5
  • Applications
  • WB, IHC-P, FC
  • Related Disease
  • Prostate Cancer; Bladder Cancer

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    FC: 1/50-1/100

Target

  • Alternative Names
  • R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
  • Long Name
  • CD82 molecule
  • Cellular Localization
  • Cell membrane
  • Post Translation Modifications
  • Glycosylation at Asn129, Asn157, and Asn198 (NX_P27701 [NX_P27701-1])
    Ubiquitination at Lys190 (NX_P27701 [NX_P27701-1])
    Modification sites at PhosphoSitePlus (P27701)
  • Function
  • Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CD82"

Recombinant Antibody

CAT Product Name Application Type
MOB-1186z Mouse Anti-CD82 Recombinant Antibody (clone 28B4) WB, FC, ICC, IHC, IP, FuncS Mouse IgG2a
MOB-1605MZ Recombinant Mouse Anti-Human CD82 Antibody (clone C-M3), PE-Conjugated FC Mouse antibody
VS3-FY322 Recombinant Mouse Anti-CD82 Antibody (clone 5D5D5) ELISA, WB, IHC, FC Mouse IgG1
VS3-FY323 Recombinant Mouse Anti-CD82 Antibody (clone 7G8G4) ELISA, IHC, FC Mouse IgG2a

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0585 Hi-Affi™ Rabbit Anti-CD82 Recombinant Antibody (clone DS585AB) FC, IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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