Rat Anti-Cd8a Recombinant Antibody (clone YTS105.18) (CAT#: HPAB-S0183-YC)

Figure 1 YTS156.7.7, but not YTS105.18, blocks MHC class I and CD8 interaction and subsequent T cell activation.

(a) Ca²⁺ mobilization in OT-I RAG^- splenocytes treated with H-2K^b tetramers loaded with the OVA peptide after pre-staining with mAbs 53.6.7 (red line), YTS105.18 (purple line) or YTS156.7.7 (green line). The tetramers were added at 1 min (arrow) and the sample re-analyzed for a further 4 min. The H-2K^b/OVA tetramer trace is taken from a 5 min time course and overlaid here as a representation of a responding population. Response to the H-2K^b/OVA tetramers in the absence of CD8 antibodies is shown (blue line), as well as response to H-2K^b/SIYR tetramers (black line) as a negative control. All traces indicate the median of the responding population. (b) Equivalent plot to show relative Ca²⁺ mobilization in OT-I RAG^- splenocytes treated with H-2K^b/OVA tetramers after pre-staining now with only the Fabs of mAbs 53.6.7 (red line), YTS105.18 (purple line) or YTS156.7.7 (green line). The baselines for all traces are normalized to the response to H-2K^b/OVA tetramers in the absence of any Fabs (blue line).

Shore, D. A., Issafras, H., Landais, E., Teyton, L., & Wilson, I. A. (2008). The crystal structure of CD8 in complex with YTS156. 7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 αβ to MHC class I. Journal of molecular biology,384(5), 1190-1202.