Rabbit Anti-CD8A Recombinant Antibody (VS3-CJ272) (CAT#: VS3-CJ272)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human CD8A

• Species Reactivity
• Human

• Applications
• WB, ICC, IF, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:1000), Immunocytochemistry/Immunofluorescence (1:50-1:200), Immunohistochemistry (1:50-1:200).

• Alternative Names
• MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8; p32; Leu2

• Gene ID
• 925

• UniProt ID
• P01732

• Cellular Localization
• Cell membrane, Membrane, Secreted

• Post Translation Modifications
• Palmitoylated, but association with CD8B seems to be more important for the enrichment of CD8A in lipid rafts.
  O-glycosylated.
  Phosphorylated in cytotoxic T-lymphocytes (CTLs) following activation.

• Protein Refseq
• NP_001139345.1; NP_001759.3; NP_741969.1

• Function
• Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody