Rabbit Anti-CD9 Recombinant Antibody (VS3-CJ782) (CAT#: VS3-CJ782)
This product is a rabbit antibody that recognizes human, mouse, and rat CD9.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/if_selected.jpg
Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/207_C1_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/207_C1_1_blue_red_green.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_B_9_5.jpg
Figure 5 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_A_7_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_A_8_5.jpg
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_A_4_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2490/6650_A_4_6.jpg
Figure 8 RNA cell line category: Cell line enhanced (CAPAN-2, RT4, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010278-CD9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010278-CD9
Figure 8 RNA cell line category: Cell line enhanced (CAPAN-2, RT4, TIME)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010278-CD9
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000010278-CD9
Specifications
- Immunogen
- Synthetic peptide within Human CD9 aa 179-228
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat CD9
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
- Gene ID
- 928
- UniProt ID
- P21926
- Sequence Similarities
- Belongs to the tetraspanin (TM4SF) family.
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids (PubMed:11959120).
Palmitoylation by ZDHHC2 regulates CD9 expression, association with other tetraspanin family proteins and function in cell adhesion (PubMed:18508921).
- Protein Refseq
- NP_001317241.1; NP_001760.1
- Function
- Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721).
Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity).
In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity).
In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480).
Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity).
Acts as a receptor for PSG17 (By similarity).
Involved in platelet activation and aggregation (PubMed:18541721).
Regulates paranodal junction formation (By similarity).
Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD9"
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-362LC | Human Anti-Human CD9 Recombinant Antibody (10E4) | FC, Inhib | Human antibody |
| TAB-363LC | Anti-Human CD9 Recombinant Antibody (11G3) | FC, WB, ELISA | Human antibody |
| TAB-364LC | Human Anti-Human CD9 Recombinant Antibody (12F3) | FC, WB, ELISA | Human antibody |
| TAB-365LC | Human Anti-Human CD9 Recombinant Antibody (3F3) | FC, WB, ELISA | Human antibody |
| TAB-366LC | Human Anti-Human CD9 Recombinant Antibody (8A3) | FC, WB, ELISA | Human antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-368LC | Mouse Anti-CD9 Recombinant Antibody (TAB-368LC) | WB | Mouse IgG |
| TAB-369LC | Mouse Anti-CD9 Recombinant Antibody (TAB-369LC) | WB, ELISA | Mouse IgG |
| TAB-368LC-S(P) | Mouse Anti-CD9 Recombinant Antibody; scFv Fragment (TAB-368LC-S(P)) | WB, ELISA | Mouse scFv |
| TAB-368LC-F(E) | Mouse Anti-CD9 Recombinant Antibody; Fab Fragment (TAB-368LC-F(E)) | WB, ELISA | Mouse Fab |
| TAB-369LC-F(E) | Mouse Anti-CD9 Recombinant Antibody; Fab Fragment (TAB-369LC-F(E)) | WB, ELISA | Mouse Fab |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-436CQ | Mouse Anti-CD9 Recombinant Antibody (clone MM2/57) | Block, FC, IHC-Fr, WB | Mouse IgG2b |
| NEUT-437CQ | Mouse Anti-CD9 Recombinant Antibody (clone MEM-61) | WB, IHC-P, FC, Block, FuncS | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0592 | Hi-Affi™ Rabbit Anti-CD9 Recombinant Antibody (clone DS592AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0081-LS-S(P) | Human Anti-CD9 Recombinant Antibody; scFv Fragment (HPAB-0081-LS-S(P)) | ELISA, Inhib | Human scFv |
| HPAB-0082-LS-S(P) | Mouse Anti-CD9 Recombinant Antibody (clone AR40A746.2.3); scFv Fragment | IP, FC, Inhib | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0081-LS-F(E) | Human Anti-CD9 Recombinant Antibody; Fab Fragment (HPAB-0081-LS-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-0082-LS-F(E) | Mouse Anti-CD9 Recombinant Antibody (clone AR40A746.2.3); Fab Fragment | IP, FC, Inhib | Mouse Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0321-CN | Rabbit Anti-CD9 Recombinant Antibody (clone CBACN-123) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-XY459 | Mouse Anti-CD9 Recombinant Antibody (clone 5G6) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-XY460 | Mouse Anti-CD9 Recombinant Antibody, FITC (clone 4AHI9a) | FC, IF | Mouse IgG1 |
| VS3-XY461 | Mouse Anti-CD9 Recombinant Antibody, PE (clone 4AHI9a) | FC, IF | Mouse IgG1 |
| VS3-XY462 | Mouse Anti-CD9 Recombinant Antibody (clone 4AHI9a) | FC, IF | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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