This product is a rabbit antibody that recognizes CDC5L.
Figure 1 Rabbit Anti-CDC5L Antibody (ZG-0607J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate
All lanes: CDC5L antibody at 1.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 93 KDa
Observed band size: 100 KDa
Figure 2 Rabbit Anti-CDC5L Antibody (ZG-0607J) in IHC
IHC image of ZG-0607J diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Rabbit Anti-CDC5L Antibody (ZG-0607J) in IHC
IHC image of ZG-0607J diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 4 Rabbit Anti-CDC5L Antibody (ZG-0607J) in IF
Immunofluorescence staining of A549 cells with ZG-0607J at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Figure 5 Rabbit Anti-CDC5L Antibody (ZG-0607J) in IP
Immunoprecipitating CDC5L in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0607J in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0607J (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3419z | Mouse Anti-CDC5L Recombinant Antibody (clone 39H10) | WB, IP, IF, ELISA | Mouse IgG1 |
MOB-2377CT | Recombinant Mouse anti-Human CDC5L Monoclonal antibody (27D23) | WB, IP, ICC, Dot | |
MRO-0333-CN | Rabbit Anti-CDC5L Recombinant Antibody (clone CBACN-134) | WB, IF, IHC | Rabbit IgG |
VS3-WK1112 | Rabbit Anti-CDC5L Recombinant Antibody (VS3-WK1112) | WB, ICC, IF, IHC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0606 | Hi-Affi™ Rabbit Anti-CDC5L Recombinant Antibody (clone DS606AB) | WB, IHC, ICC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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