Mouse Anti-CDH3 Recombinant Antibody (clone TSP7) (CAT#: PABC-251)

Figure 1 ELISA

Binding of antibodies to P-cadherin on cells analyzed by cell ELISA. Plots of TSP5, TSP7, and TSP11 are shown in black, red, and cyan respectively. Each plot represents the mean ± SD.

Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.

Figure 2 Time-dependent inhibition of the cell adhesion by TSP7.

Live cell imaging using CHO cells stably expressing human P-cadherin WT-mGFP or K14E-mGFP in the absence and presence of TSP7. Images were obtained with an In Cell Analyzer 2000 instrument (GE Healthcare) with a 60 × 0.7 NA objective lens. Scale bar represents 10 μm. The arrow indicates a typical cell-cell junction region. (b) Time-dependent changes in the intensity of the dense fluorescent spots (puncta) at cell-cell boundaries. Plots for P-cadherin WT and K14E are shown as black and gray lines, respectively. Plots are the average ± SD.

Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.

Figure 3 Kinetic parameters by SPR.

Kudo, S., Caaveiro, J. M., Nagatoishi, S., Miyafusa, T., Matsuura, T., Sudou, Y., & Tsumoto, K. (2017). Disruption of cell adhesion by an antibody targeting the cell-adhesive intermediate (X-dimer) of human P-cadherin. Scientific reports, 7, 39518.