Rabbit Anti-CDK4 Recombinant Antibody (clone 8F2) (CAT#: ZG-0612J)
This product is a rabbit antibody that recognizes CDK4.
Specific Inquiry
WB
Figure 1 Rabbit Anti-CDK4 Antibody (ZG-0612J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate
All lanes: CDK4 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 21 kDa
Observed band size: 34 kDa
IP
Figure 2 Rabbit Anti-CDK4 Antibody (ZG-0612J) in IP
Immunoprecipitating CDK4 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0612J in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500)
Lane 2:ZG-0612J(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human CDK4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDK4
- Species Reactivity
- Human
- Clone
- 8F2
- Applications
- ELISA, WB, IP
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunoprecipitation.
WB:1:500-1:5000, IP:1:200-1:1000
Target
- Alternative Names
- CMM3; PSK-J3; cyclin-dependent kinase 4; cell division protein kinase 4
- Gene ID
- 1019
- UniProt ID
- P11802
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CDK4"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2055z | Mouse Anti-CDK4 Recombinant Antibody (clone 23A2) | WB, ICC, IF, IHC | Mouse IgG |
| MRO-0339-CN | Mouse Anti-CDK4 Recombinant Antibody (clone 5-C3-G3) | WB, IF, IHC | Mouse IgG2c |
| MRO-0340-CN | Rabbit Anti-CDK4 Recombinant Antibody (clone CBACN-138) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-0341-CN | Rabbit Anti-CDK4 Recombinant Antibody (clone CBACN-139) | WB, IF, IP | Rabbit IgG |
| ZG-0404F | Mouse Anti-Cdk4 Recombinant Antibody (ZG-0404F) | WB | Mouse IgG |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0113MZ | Chicken Anti-CDK4 Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0630 | Hi-Affi™ Rabbit Anti-CDK4 Recombinant Antibody (clone DS630AB) | ICC, IF, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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