Mouse Anti-CHGA Recombinant Antibody (clone ABT-CGA) (CAT#: VS3-CJ571)

• Immunogen
• Synthesized peptide derived from human Chromogranin A

• Host Species
• Mouse

• Type
• Mouse IgG3, κ

• Specificity
• Human CHGA

• Species Reactivity
• Human

• Clone
• ABT-CGA

• Applications
• IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• CGA; PHE5; PHES

• Gene ID
• 1113

• UniProt ID
• P10645

• Sequence Similarities
• Belongs to the chromogranin/secretogranin protein family.

• Cellular Localization
• Cytoplasmic vesicle, Secreted

• Post Translation Modifications
• Sulfated on tyrosine residues and/or contains sulfated glycans.
  O-glycosylated with core 1 or possibly core 8 glycans.
  Proteolytic processing gives rise to an additional longer form of catestatin (residues 358-390) which displays a less potent catecholamine release-inhibitory activity (PubMed:10781584).
  Plasmin-mediated proteolytic processing can give rise to additional shorter and longer forms of catestatin peptides (PubMed:17991725).

• Protein Refseq
• NP_001266.1; NP_001288619.1

• Function
• Pancreastatin:
  Strongly inhibits glucose induced insulin release from the pancreas.
  Catestatin:
  Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220).
  Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458).
  Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543).
  Acts as a potent scavenger of free radicals in vitro (PubMed:24723458).
  May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).
  Serpinin:
  Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Isotype Control

• MOB-205CQ
• Mouse IgG3, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1470-YJ
• Goat Anti-Mouse IgG3 Secondary antibody

• 2RD-A1569-YJ
• Rabbit Anti-Mouse IgG3 Secondary antibody (Clone RM218)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS