Human Anti-Collagen Recombinant Antibody (clone HUIV26) (CAT#: HPAB-1166WJ)
This product is a humanized antibody targeting collagen. This antibody has specific binding activity for a cryptic collagen epitope, including inhibiting angiogenesis, tumor growth, and metastasis.
Specific Inquiry
ELISA
Figure 1 Mab HUIV26 reactivity with denatured/proteolyzed collagen IV in solid phase ELISA.
Microtiter wells were coated with triple helical collagen IV at 25 μg/ml.
Xu, J., Rodriguez, D., Petitclerc, E., Kim, J. J., Hangai, M., Yuen, S. M., ... & Brooks, P. C. (2001). Proteolytic exposure of a cryptic site within collagen type IV is required for angiogenesis and tumor growth in vivo. The Journal of cell biology, 154(5), 1069-1080.
IF
Figure 2 Exposure of the HUIV26 cryptic site within human tissue by proteolysis.
Normal human retinal tissue or retina from subjects with diabetic retinopathy were costained with Mab HUIV26 and a polyclonal antibody to factor VIII–related antigen. Green indicates human blood vessels and red indicates HUIV26 cryptic sites.
Xu, J., Rodriguez, D., Petitclerc, E., Kim, J. J., Hangai, M., Yuen, S. M., ... & Brooks, P. C. (2001). Proteolytic exposure of a cryptic site within collagen type IV is required for angiogenesis and tumor growth in vivo. The Journal of cell biology, 154(5), 1069-1080.
DB
Figure 3 Exposure of the HUIV26 cryptic epitope is associated with the expression and activation of MMP-2 in vivo.
Dot blot of total CAM lysates. Total collagen IV (triple helical and denatured) was detected with a polyclonal antibody to both native and denatured collagen IV. Denatured collagen IV was detected with Mab HUIV26.
Inhib
Figure 4 Effects of purified Mab HUIV26 on angiogenesis in vivo.
Quantification of the area of neovascularization within rat corneas. Data bars represent the mean area of neovascularization from the limbus to hydron pellet.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Clone
- HUIV26
- Applications
- ELISA, IF, DB, Inhib
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Collagen antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunofluorescence, Dot Blot, Inhibition.
ELISA: 1 μg/ml.
Target
- Alternative Names
- Collagen
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone HUIV26"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-228 | Recombinant Anti-Collagen Antibody | WB, FC, FuncS | IgG |
| MHH-228 | Recombinant Human Anti-Collagen Antibody | WB, Dot, FuncS | IgG |
| MOB-0299MZ | Recombinant Mouse Anti-Human Collagen IV Antibody (clone DIV 23 (2)) | IHC | Mouse antibody |
| HPAB-1167WJ | Human Anti-Collagen Recombinant Antibody (clone HUI77) | ELISA, Inhib | Humanized IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-228-F(E) | Recombinant Anti-Collagen Antibody Fab Fragment | Neut, IF, FuncS | Fab |
| MHH-228-F(E) | Recombinant Human Anti-Collagen Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| HPAB-1166WJ-F(E) | Human Anti-Collagen Recombinant Antibody (clone HUIV26); Fab Fragment | ELISA | Humanized Fab |
| HPAB-1167WJ-F(E) | Human Anti-Collagen Recombinant Antibody (clone HUI77); Fab Fragment | ELISA | Humanized Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-228-S(P) | Recombinant Anti-Collagen Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| MHH-228-S(P) | Recombinant Human Anti-Collagen Antibody scFv Fragment | IF, FC, Biosensors, FuncS | scFv |
| HPAB-1166WJ-S(P) | Human Anti-Collagen Recombinant Antibody (clone HUIV26); scFv Fragment | ELISA | Humanized scFv |
| HPAB-1167WJ-S(P) | Human Anti-Collagen Recombinant Antibody (clone HUI77); scFv Fragment | ELISA | Humanized scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-182CL | Human Anti-Collagen Recombinant Antibody (TAB-182CL) | ELISA | Human IgG |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-050MZ | Human Anti-Collagen Recombinant Antibody (TAB-050MZ) | ELISA, IHC, IF | Human IgG |
| TAB-051MZ | Human Anti-Collagen Recombinant Antibody (TAB-051MZ) | ELISA, IHC, IF | Human IgG |
| TAB-050MZ-S(P) | Human Anti-Collagen Recombinant Antibody; scFv Fragment (TAB-050MZ-S(P)) | ELISA, IHC, IF | Human scFv |
| TAB-050MZ-F(E) | Human Anti-Collagen Recombinant Antibody; Fab Fragment (TAB-050MZ-F(E)) | ELISA, IHC, IF | Human Fab |
| TAB-051MZ-F(E) | Human Anti-Collagen Recombinant Antibody; Fab Fragment (TAB-051MZ-F(E)) | ELISA, IHC, IF | Human Fab |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-052MZ | Human Anti-Collagen Recombinant Antibody (TAB-052MZ) | WB | Chimeric (mouse/human) IgG1 |
| TAB-052MZ-S(P) | Mouse Anti-Collagen Recombinant Antibody; scFv Fragment (TAB-052MZ-S(P)) | WB | Mouse scFv |
| TAB-052MZ-F(E) | Human Anti-Collagen Recombinant Antibody; Fab Fragment (TAB-052MZ-F(E)) | WB | Chimeric (mouse/human) Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-057MZ | Anti-Human Collagen Recombinant Antibody (20A10) | ELISA | |
| TAB-057MZ-S(P) | Anti-Human Collagen Recombinant Antibody scFv Fragment (20A10) | ELISA | |
| TAB-057MZ-F(E) | Anti-Human Collagen Recombinant Antibody Fab Fragment (20A10) | ELISA |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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