Mouse Anti-CRTAM Recombinant Antibody (clone 17B2) (CAT#: HPAB-1089-FY)

Figure 1 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SDS-PAGE

SDS-PAGE analysis of HPAB-1089-FY in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in SEC-HPLC

The purity of HPAB-1089-FY was greater than 98% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in DB

Dot Blot analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag).

HPAB-1089-FY incubation concentration: 2 μg/mL.

HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 4 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in WB

Western blot analysis of HPAB-1089-FY was performed by loading human CRTAM protein, His tag.

HPAB-1089-FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L).

Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)

Figure 5 Mouse Anti-CRTAM Recombinant Antibody (HPAB-1089-FY) in ELISA

ELISA analysis of HPAB-1089-FY was performed by coating with human CRTAM protein (His tag). Then blocked with BSA and incubated with HPAB-1089-FY. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.