This product is a recombinant antibody that recognizes CXCR4. The monoclonal antibody Ab124 reacts with the epitope of CXCR4. Ab124 can inhibit SDF-1 induced chemotaxis in human breast cancer line MDA-MB-231.
Figure 1 Anti-CXCR4 scFv-binding assays.
ELISA-binding assays were performed to measure anti-CXCR4 scFv (Ab124; Ab125) binding to PC-3 breast cancer cells.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
Figure 2 Anti-CXCR4 scFv binding to PCa cells.
Flow cytometric analysis was performed using a three-step procedure. Cells were first incubated with Ab16, Ab124, or Ab125 (10 μg/ml) or without scFv, followed by anti-HSV Tag mAb (0.5 μg/ml), then fluorescent antimouse IgG antibody.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
Figure 3 Anti-CXCR4 scFv inhibit CXCL12-induced calcium flux.
The effects of Ab124 and Ab125 on calcium mobilization in PCa cells was determined. PC-3 and LNCaP cells were preincubated with Ab16, Ab124, or Ab125 (10 μg/ml) for 30 min, then exposed to CXCL12 (100 ng/ml) for measurement of calcium flux.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
Figure 4 Anti-CXCR4 scFv inhibit CXCL12-induced chemotaxis.
Chemotaxis assays were performed to determine the inhibitory effects of Ab124 and Ab125 on CXCL12-induced migration of PCa cells and control T cells. PC-3 cells were preincubated with Ab16, Ab124, or Ab125 (10 μg/ml) for 30 min at 37°C, then incubated in Transwell migration chambers containing CXCL12 (400 ng/ml) for 18–24 h.
Vaday, G. G., Hua, S. B., Peehl, D. M., Pauling, M. H., Lin, Y. H., Zhu, L., ... & Zucker, S. (2004). CXCR4 and CXCL12 (SDF-1) in prostate cancer: inhibitory effects of human single chain Fv antibodies. Clinical cancer research, 10(16), 5630-5639.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0011CL | Anti-Human CXCR4 Recombinant Antibody (414H5) | FC, IHC, Block, Inhib, FuncS | |
TAB-0012CL | Anti-Human CXCR4 Recombinant Antibody (515H7) | Inhib, FC | |
TAB-0011CL-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (414H5) | FC, IHC, Block, Inhib, FuncS | |
TAB-0012CL-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (515H7) | FC, IHC, Block, Inhib, FuncS | |
TAB-143MZ | Mouse Anti-CXCR4 Recombinant Antibody (TAB-143MZ) | Cyt, FuncS, Inhib | Mouse lgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-146MZ | Human Anti-CXCR4 Recombinant Antibody (TAB-146MZ) | Cyt, FuncS, Inhib | Humanized IgG1 |
TAB-147MZ | Human Anti-CXCR4 Recombinant Antibody (TAB-147MZ) | Cyt, FuncS, Inhib | Humanized IgG1 |
TAB-146MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-146MZ-S(P)) | cAMP Assay | Humanized scFv |
TAB-147MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-147MZ-S(P)) | cAMP Assay | Humanized scFv |
TAB-148MZ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (TAB-148MZ-S(P)) | cAMP Assay | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-150MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (CF172) | FC | Human antibody |
TAB-151MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (5C9) | WB, ELISA | Human antibody |
TAB-152MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (5E1) | WB, ELISA | Human antibody |
TAB-153MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (7C8) | WB, ELISA | Human antibody |
TAB-154MZ-F(E) | Anti-Human CXCR4 Recombinant Antibody Fab Fragment (6C7) | WB, ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0152MZ | Chicken Anti-CXCR4 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-684CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 12G5) | FC, CyTOF®, Neut | Mouse IgG2a |
NEUT-685CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 12G5R) | FC, Neut | Mouse IgG2a |
NEUT-687CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL046) | FC, IHC, Neut | Mouse IgG2 |
NEUT-688CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL470) | FC, CyTOF®, Neut | Mouse IgG2b |
NEUT-689CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone CBL783) | Neut | Mouse IgG2a |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-686CQ | Mouse Anti-CXCR4 Recombinant Antibody (clone 44716.111) | FC, ICC, IF, IHC-P, Inhib | Mouse IgG2b |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0889 | Hi-Affi™ Rabbit Anti-CXCR4 Recombinant Antibody (clone DS889AB) | WB, IHC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0044-YC | Human Anti-CXCR4 Recombinant Antibody (clone hz515H7VH1D76NVL2) | Block, Cyt, FC | Humanized IgG1, κ |
HPAB-0045-YC | Human Anti-CXCR4 Recombinant Antibody (HPAB-0045-YC) | FC, Cyt | Humanized IgG |
HPAB-0374LY | Human Anti-CXCR4 Recombinant Antibody (HPAB-0374LY) | ELISA | Human IgG |
MRO-1796-CN | Rabbit Anti-CXCR4 Polyclonal Antibody (MRO-1796-CN) | WB, IF, IHC, FC | Rabbit IgG |
HPAB-0329-WJ | Mouse Anti-CXCR4 Recombinant Antibody (HPAB-0329-WJ) | ELISA, FC, IHC | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0659-CN-F(E) | Human Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0659-CN-F(E)) | FC, Neut | Human Fab |
HPAB-0660-CN-F(E) | Human Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0660-CN-F(E)) | FC, Neut | Human Fab |
HPAB-0661-CN-F(E) | Human Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0661-CN-F(E)) | FC, Neut | Human Fab |
HPAB-0374LY-F(E) | Human Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0374LY-F(E)) | ELISA | Human Fab |
HPAB-0329-WJ-F(E) | Mouse Anti-CXCR4 Recombinant Antibody; Fab Fragment (HPAB-0329-WJ-F(E)) | ELISA, FC, IHC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-H72 | Afuco™ Anti-CXCR4 ADCC Recombinant Antibody (Ulocuplumab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0685-WJ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (HPAB-0685-WJ-S(P)) | FC | Human scFv |
HPAB-0686-WJ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (HPAB-0686-WJ-S(P)) | FC | Human scFv |
HPAB-0687-WJ-S(P) | Human Anti-CXCR4 Recombinant Antibody; scFv Fragment (HPAB-0687-WJ-S(P)) | FC | Human scFv |
HPAB-1842-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone Ab124); scFv Fragment | Inhib | Human scFv |
HPAB-1843-FY-F(E) | Human Anti-CXCR4 Recombinant Antibody (clone Ab125); scFv Fragment | Inhib | Human scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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