Mouse Anti-CYCS Recombinant Antibody (clone 4B10) (CAT#: ZG-0221J)
This product is a mouse antibody that recognizes CYCS.
Specific Inquiry
IF
Figure 1 Mouse Anti-CYCS Recombinant Antibody (clone 4B10) in IF
Immunofluorescence analysis of Hela cell. 1, c-Myc Polyclonal Antibody (red) was diluted at 1:200(4°C overnight). CYCS Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 AS0784 was diluted at 1:1000 (room temperature, 50min).
IF
Figure 2 Mouse Anti-CYCS Recombinant Antibody (clone 4B10) in IF
Immunofluorescence analysis of Human-liver-cancer tissue. 1.CYCS Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-CYCS Recombinant Antibody (clone 4B10) in IHC
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.CYCS Monoclonal Antibody was diluted at 1:200 (4°C, overnight).2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min).3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-CYCS Recombinant Antibody (clone 4B10) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CYCS Monoclonal Antibody was diluted at 1:200 (4°C, overnight).2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min).3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).Negative control was used by secondary Antibody only.
WB
Figure 5 Mouse Anti-CYCS Recombinant Antibody (clone 4B10) in WB
Western blot analysis of lysates from 1) Hela, 2) 293T, 3) 3T3, cells, :reen:primary Antibody was diluted at 1:1000, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C 1 hour. GAPDH Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C1 hour.
WB
Figure 6 Mouse Anti-CYCS Recombinant Antibody (clone 4B10) in WB
Western blot analysis of 1) Hela, 2) 293T, 3) 3T3, 4) Mouse Liver Tissue, 5) Rat Liver Tissue, 6) Rat Kidney Tissue using CYCS Monoclonal Antibody.
Specifications
- Immunogen
- Recombinant Protein of CYCS
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CYCS
- Species Reactivity
- Human, Mouse, Rat, Chicken
- Clone
- 4B10
- Applications
- WB, IF/ICC, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CYCS"
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-258-S(P) | Recombinant Anti-human CYCS Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| MHH-258-S(P) | Recombinant Human Anti-human CYCS Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| PSBC-073 | Mouse Anti-CYCS Recombinant Antibody (clone E8); scFv Fragment | WB, FuncS | Mouse scFv |
| FAMAB-0116JF-S(P) | Mouse Anti-CYCS Recombinant Antibody (clone 5A3.B6); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0257JF-S(P) | Mouse Anti-CYCS Recombinant Antibody (clone 6A7.E5); scFv Fragment | ELISA, FuncS | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-258 | Recombinant Human Anti-human CYCS Antibody | WB, IP, FuncS | IgG |
| FAMAB-0243JF | Mouse Anti-CYCS Recombinant Antibody (clone 7E4) | ELISA, FuncS | Mouse IgG1 |
| FAMAB-0253JF | Mouse Anti-CYCS Recombinant Antibody (clone 1G1.E9) | ELISA, FuncS | Mouse IgG |
| FAMAB-0254JF | Mouse Anti-CYCS Recombinant Antibody (clone 7D4.H4) | ELISA, FuncS | Mouse IgG |
| FAMAB-0255JF | Mouse Anti-CYCS Recombinant Antibody (clone 2F8.C10) | ELISA, FuncS | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-258-F(E) | Recombinant Human Anti-human CYCS Antibody Fab Fragment | WB, IF, FuncS | Fab |
| PFBC-073 | Mouse Anti-CYCS Recombinant Antibody (clone E8); Fab Fragment | WB, FuncS | Mouse Fab |
| FAMAB-0243JF-F(E) | Mouse Anti-CYCS Recombinant Antibody (clone 7E4); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0253JF-F(E) | Mouse Anti-CYCS Recombinant Antibody (clone 1G1.E9); Fab Fragment | ELISA, FuncS | Mouse Fab |
| FAMAB-0254JF-F(E) | Mouse Anti-CYCS Recombinant Antibody (clone 7D4.H4); Fab Fragment | ELISA, FuncS | Mouse Fab |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0006MZ | Recombinant Chicken Anti-Cytochrome c IgY (1E4) | WB | Chicken antibody |
MHC Tetramer for Other Applications
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-698CQ | Mouse Anti-CYCS Recombinant Antibody (clone 6H2.B4) | IP, Neut | Mouse IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0895 | Hi-Affi™ Rabbit Anti-CYCS Recombinant Antibody (clone DS895AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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