Mouse Anti-DERP2 Recombinant Antibody (clone H10) (CAT#: FAMAB-1836CQ)

Figure 1 Basophil degranulation at different concentrations of total rIgE.

FACS results of human basophils sensitized with total rIgE concentrations of 3, 1, 0.2, 0.04, 0.008, and 0.0016 μg/mL, respectively, are shown. The composition of the rIgE mixture was kept constant with 20% rDer p 2-specific rIgE consisting of equimolar quantities (6.67% each) of 3 rIgE clones (G, H10, and H12) recognizing nonoverlapping Der p 2 epitopes and 80% non-Der p 2-specific rIgE (anti-tox). Upper left, Schematic representation of Der p 2 epitope specificity and affinity of rIgE clones G, H10, and H12.

Christensen, L. H., Holm, J., Lund, G., Riise, E., & Lund, K. (2008). Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge. Journal of Allergy and Clinical Immunology, 122(2), 298-304.

Figure 2 Basophil degranulation at different concentrations of Der p 2-specific rIgE relative to non-Der p 2-specific rIgE.

FACS results of human basophils sensitized with different concentrations of Der p 2-specific rIgE (srIgE) relative to non-Der p 2-specific rIgE (non-srIgE) are shown. The composition of the rIgE mixtures consisted of equimolar quantities of 3 rIgE clones (E, H10, and H12) recognizing nonoverlapping Der p 2 epitopes and variable concentrations of non-Der p 2-specific rIgE (anti-tox). The total rIgE concentration was kept constant at 1 μg/mL.

Christensen, L. H., Holm, J., Lund, G., Riise, E., & Lund, K. (2008). Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge. Journal of Allergy and Clinical Immunology, 122(2), 298-304.

Figure 3 IgE affinities for rDer p 2.

A-C, Examples of affinity determinations of rIgE clones binding rDer p 2 with high, medium, and low affinity, respectively, as obtained by using surface plasmon resonance experiments. Only the relevant part from where rDer p 2 is injected is shown. D, Summary of affinities obtained of all rIgE clones.

Christensen, L. H., Holm, J., Lund, G., Riise, E., & Lund, K. (2008). Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge. Journal of Allergy and Clinical Immunology, 122(2), 298-304.