Human Anti-DNA Recombinant Antibody (clone O-81) (CAT#: FAMAB-0046WJ)
This product is a human IgM antibody targeting single stranded DNA derived from a patient with active lupus nephritis.
Specific Inquiry
ELISA
Figure 1 Cross-reactivity of lupus anti-DNA Abs to Herp.
Binding activity of O-81 mAb (1.0 μg/ml) to ssDNA (open boxes), dsDNA (closed circles), and sHerp (closed triangles) in direct binding ELISA.
Hirabayashi, Y., Oka, Y., Ikeda, T., Fujii, H., Ishii, T., Sasaki, T., & Harigae, H. (2010). The endoplasmic reticulum stress-inducible protein, Herp, is a potential triggering antigen for anti-DNA response. The Journal of Immunology, 184(6), 3276-3283.
Inhib
Figure 2 Inhibition of anti-DNA production with transfect pLNCX containing V3-7 ribozyme.
O-81 cells was transfected with pLNCX containing V3-7 ribozyme or with pLNCX alone. After being washed, the cells were cultured in culture medium containing geneticin for the indicated days and was tested for the anti-DNA activity in the culture supernatant.
Suzuki, Y., Funato, T., Munakata, Y., Sato, K., Hirabayashi, Y., Ishii, T., ... & Sasaki, T. (2000). Chemically modified ribozyme to V gene inhibits anti-DNA production and the formation of immune deposits caused by lupus lymphocytes. The Journal of Immunology, 165(10), 5900-5905.
Specifications
- Host Species
- Human
- Type
- Human IgM
- Specificity
- Single-stranded DNA
- Clone
- O-81
- Applications
- WB, ELISA, Inhib
- Related Disease
- Active lupus nephritis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The DNA antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Inhibition.
Target
- Alternative Names
- DNA; Deoxyribonucleic acid; polynucleotides; double heliX
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-074 | Mouse Anti-DNA Recombinant Antibody (PABL-074) | ELISA, WB, FuncS | Mouse IgG |
| PABL-075 | Mouse Anti-DNA Recombinant Antibody (clone ED10) | ELISA, WB, FuncS | Mouse IgG |
| FAMAB-0075CQ | Mouse Anti-DNA Recombinant Antibody (clone 16-13) | ELISA | Mouse IgG2a |
| FAMAB-0076CQ | Mouse Anti-DNA Recombinant Antibody (clone 1A11) | ELISA | Mouse IgG2b |
| FAMAB-0077CQ | Mouse Anti-DNA Recombinant Antibody (clone 1E81) | ELISA | Mouse IgG2a |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-048 | Recombinant Mouse Anti-DNA Antibody | WB, ELISA, FuncS | IgG |
| PABX-048-F (E) | Recombinant Mouse Anti-DNA Antibody Fab Fragment | WB, ELISA, FuncS | Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-048-S (P) | Recombinant Mouse Anti-DNA Antibody scFv Fragment | WB, ELISA, FuncS | scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0075CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 16-13); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0076CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 1A11); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0077CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 1E81); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0078CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 2F2); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0079CQ-F(E) | Mouse Anti-DNA Recombinant Antibody (clone 3E12); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0271-YJ-S(P) | Mouse Anti-DNA Recombinant Antibody; scFv Fragment (HPAB-0271-YJ-S(P)) | FuncS | Mouse scFv |
| FAMAB-0036WJ-S(P) | Human Anti-DNA Recombinant Antibody (clone T33-17); scFv Fragment | WB | Human scFv |
| FAMAB-0066WJ-S(P) | Human Anti-DNA Recombinant Antibody (clone B4E7); scFv Fragment | WB | Human scFv |
| FAMAB-0067WJ-S(P) | Human Anti-DNA Recombinant Antibody (clone D2C10); scFv Fragment | WB | Human scFv |
| FAMAB-0068WJ-S(P) | Human Anti-DNA Recombinant Antibody (clone C6B2); scFv Fragment | WB | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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