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  • Rabbit Anti-DPF2 Recombinant Antibody (clone R39-2M-4)

Rabbit Anti-DPF2 Recombinant Antibody (clone R39-2M-4) (CAT#: VS3-FY2168)

Clone R39-2M-4 is a rabbit IgG recombinant monoclonal antibody that reacts with human DPF2 protein, a calculated weight of 44 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R39-2M-4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200), immunoprecipitation (dilution range 1:50).


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Specific Inquiry
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
Figure 2 IHC staining of human salivary gland
(Immunohistochemical staining of human salivary gland shows weak cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_1_1_selected.jpg
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_8_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_3.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_8_5.jpg
Figure 6 Testis
Figure 6 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_A_4_6.jpg
Figure 7 Skin
Figure 7 Skin
(Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20880/47701_B_7_1.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133884-DPF2

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat DPF2
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R39-2M-4
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • The calculated weight of the target protein is approximately 44 kDa, while the observed molecular weight stands at 44kDa.
  • Related Disease
  • Cancer

Product Property

  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/100-1/200
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/50

Target

  • Alternative Names
  • Double PHD Fingers 2; D4, Zinc And Double PHD Fingers Family 2; Apoptosis Response Zinc Finger Protein; BRG1-Associated Factor 45D; Protein Requiem; BAF45D; UBID4
  • Sequence Similarities
  • Belongs to the requiem/DPF family.
  • Cellular Localization
  • Cytoplasm, Nucleus
  • Post Translation Modifications
  • Ubiquitination at Lys59 and Lys108 (NX_Q92785 [NX_Q92785-1])
    Modification sites at PhosphoSitePlus (Q92785)
    Glycosylation from GlyGen (Q92785) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Plays an active role in transcriptional regulation by binding modified histones H3 and H4.
    Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells.
    Might also have a role in the development and maturation of lymphoid cells (By similarity).
    Involved in the regulation of non-canonical NF-kappa-B pathway.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "DPF2"

Recombinant Antibody

CAT Product Name Application Type
MOB-0597CT Recombinant Mouse anti-Human DPF2 Monoclonal antibody (3394D2b) Dot, WB

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1038 Hi-Affi™ Rabbit Anti-DPF2 Recombinant Antibody (clone DS1038AB) WB, IHC, ICC, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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