This recombinant monoclonal antibody to Ebola virus glycoprotein is a human monoclonal antibody that can be used for applications: ELISA, Neut.
Figure 1 Immunoprecipitation (IP) of soluble GP ectodomain containing or lacking the mucin-like domain (GPΔMuc) by mAb100, mAb114 or isotype control.
Binding and input were analyzed using immunoblotting for GP1. * Represents a GP1 degradation product present only in mucin-containing GP. (n=3, representative image shown)
Misasi, J., Gilman, M. S., Kanekiyo, M., Gui, M., Cagigi, A., Mulangu, S., ... & McLellan, J. S. (2016). Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 351(6279), 1343-1346.
Figure 2 GPΔMuc was incubated with the indicated antibodies followed by cleavage at pH 5.5 by Cat L at 37 °C.
Samples were removed at 5 minute intervals and analyzed by immunoblot for GP1. (n=3, representative image shown)
Misasi, J., Gilman, M. S., Kanekiyo, M., Gui, M., Cagigi, A., Mulangu, S., ... & McLellan, J. S. (2016). Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 351(6279), 1343-1346.
Figure 3 Immunoprecipitation of GPΔMuc and GPTHL by the indicated antibodies.
Samples were analyzed by immunoblot for GP1. (n=3, representative image shown)
Misasi, J., Gilman, M. S., Kanekiyo, M., Gui, M., Cagigi, A., Mulangu, S., ... & McLellan, J. S. (2016). Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 351(6279), 1343-1346.
Figure 4 Class averages of single particles from negative-stain electron micrographs of Fab114 in complex with GPΔMuc and GPTHL.
Misasi, J., Gilman, M. S., Kanekiyo, M., Gui, M., Cagigi, A., Mulangu, S., ... & McLellan, J. S. (2016). Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 351(6279), 1343-1346.
Figure 5 Binding kinetics of GPΔMuc or GPTHL with Fab114, 13C6 or monomeric domain C of NPC1 (NPC1-dC) at the indicated pH as measured by biolayer interferometry.
Equilibrium dissociation constants (KD) are plotted on a negative log scale. * indicates no binding. (n=2, representative experiment shown)
Misasi, J., Gilman, M. S., Kanekiyo, M., Gui, M., Cagigi, A., Mulangu, S., ... & McLellan, J. S. (2016). Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 351(6279), 1343-1346.
Figure 6 Inhibition of NPC1-dC binding to GPTHL by competitor proteins (NPC1-dC) or antibodies (mAb100, mAb114, 13C6, KZ52, isotype control) was determined by biolayer interferometry.
Dashed line represents 60% inhibition of binding. (n=3, representative experiment shown)
Misasi, J., Gilman, M. S., Kanekiyo, M., Gui, M., Cagigi, A., Mulangu, S., ... & McLellan, J. S. (2016). Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science, 351(6279), 1343-1346.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MRO-927LC | Mouse Anti-EBOV GP Recombinant Antibody (MRO-927LC) | ELISA | Mouse IgG1, κ |
MRO-927LC-S(P) | Mouse Anti-EBOV GP Recombinant Antibody (MRO-927LC-S(P)); scFv Fragment | ELISA | Mouse scFv |
MRO-927LC-F(E) | Mouse Anti-EBOV GP Recombinant Antibody (MRO-927LC-F(E)); Fab Fragment | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-936LC | Human Anti-EBOV GP Recombinant Antibody (MRO-936LC) | ELISA | Human IgG1, κ |
MRO-917LC-S(P) | Anti-EBOV GP Recombinant Antibody scFv Fragment (EVB100) | ELISA | Human antibody |
MRO-918LC-S(P) | Anti-EBOV GP Recombinant Antibody scFv Fragment (EVB166) | ELISA | Human antibody |
MRO-919LC-S(P) | Anti-EBOV GP Recombinant Antibody scFv Fragment (EVB167) | ELISA | Human antibody |
MRO-920LC-S(P) | Anti-EBOV GP Recombinant Antibody scFv Fragment (EVB165) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-937LC | Anti-EBOV GP Recombinant Antibody (CAN9G1) | ELISA, WB | Mouse antibody |
MRO-938LC | Anti-EBOV GP Recombinant Antibody (CAN8G1) | ELISA, WB | Mouse antibody |
MRO-939LC | Anti-EBOV GP Recombinant Antibody (CAN7G1) | ELISA, WB | Mouse antibody |
MRO-900LC-S(P) | Anti-EBOV GP Recombinant Antibody scFv Fragment (13F6) | ELISA | Mouse antibody |
MRO-901LC-S(P) | Anti-EBOV GP Recombinant Antibody scFv Fragment (6D8) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0983LC | Recombinant Mouse Anti-EBOV GP Antibody (133/3.16) | ELISA, FuncS | IgG1 |
EPAF-0213CQ | Recombinant Mouse Anti-EBOV GP Antibody (226/8.1) | Neut | IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0106-YC-F(E) | Macaque Anti-EBOV GP Recombinant Antibody; Fab Fragment (HPAB-N0106-YC-F(E)) | ELISA, FC, Neut | Macaque Fab |
HPAB-0734YY-F(E) | Human Anti-EBOV GP Recombinant Antibody; Fab Fragment (HPAB-0734YY-F(E)) | ELISA, Neut | Human Fab |
HPAB-0735YY-F(E) | Human Anti-EBOV GP Recombinant Antibody; Fab Fragment (HPAB-0735YY-F(E)) | ELISA, Neut | Humanized Fab |
HPAB-0736YY-F(E) | Human Anti-EBOV GP Recombinant Antibody; Fab Fragment (HPAB-0736YY-F(E)) | ELISA, Neut | Humanized Fab |
HPAB-0737YY-F(E) | Human Anti-EBOV GP Recombinant Antibody; Fab Fragment (HPAB-0737YY-F(E)) | ELISA, Neut | Humanized Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0411-FY | Human Anti-EBOV GP Recombinant Antibody (HPAB-0411-FY) | ELISA, FC, FuncS, Neut | Human IgG |
HPAB-0412-FY | Human Anti-EBOV GP Recombinant Antibody (HPAB-0412-FY) | ELISA, FC, FuncS, Neut | Human IgG |
HPAB-0413-FY | Human Anti-EBOV GP Recombinant Antibody (HPAB-0413-FY) | ELISA, FC, Neut | Human IgG |
HPAB-1098WJ | Mouse Anti-EBOV GP Recombinant Antibody (HPAB-1098WJ) | ELISA, WB | Mouse IgG, κ |
HPAB-0734YY | Human Anti-EBOV GP Recombinant Antibody (HPAB-0734YY) | ELISA, Neut | Human IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0411-FY-S(P) | Human Anti-EBOV GP Recombinant Antibody; scFv Fragment (HPAB-0411-FY-S(P)) | ELISA, Neut | Human scFv |
HPAB-0412-FY-S(P) | Human Anti-EBOV GP Recombinant Antibody; scFv Fragment (HPAB-0412-FY-S(P)) | ELISA, Neut | Human scFv |
HPAB-0413-FY-S(P) | Human Anti-EBOV GP Recombinant Antibody; scFv Fragment (HPAB-0413-FY-S(P)) | ELISA, Neut | Human scFv |
HPAB-0734YY-S(P) | Human Anti-EBOV GP Recombinant Antibody; scFv Fragment (HPAB-0734YY-S(P)) | ELISA, Neut | Human scFv |
PABJ-0059-S(P) | Human Anti-EBOV GP Recombinant Antibody (clone 1T0227); scFv Fragment | ELISA | Human scFv |
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