Mouse Anti-EGF Recombinant Antibody (ZG-0281J) (CAT#: ZG-0281J)

This product is a mouse antibody that recognizes EGF.


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WB

Figure 1 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in WB

Figure 1 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in WB

Western blot detection of EGFR in HCC827,A549 and MDA-MB-468 cell lysates using EGFR mouse mAb(dilution 1:1000). Predicted band size:134 Kda.Observed band size:175KDa.

ICC

Figure 2 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Figure 2 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).

IP

Figure 3 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IP

Figure 3 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IP

Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.

IHC

Figure 4 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Figure 4 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.

ICC

Figure 5 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Figure 5 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).

IHC

Figure 6 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Figure 6 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Immunohistochemical analysis of paraffin-embedded Colorectal cancer using EGF Receptor (3F12) mouse mAb (1/800 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).


Specifications

  • Immunogen
  • Purified recombinant human EGFR protein fragments expressed in E.coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human EGF
  • Species Reactivity
  • Human
  • Applications
  • WB, ICC, IHC

Product Property

  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunocytochemistry, Immunohistochemistry.

Target

  • Alternative Names
  • EGF; URG; HOMG4; pro-epidermal growth factor; beta-urogastrone

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "EGF"

Neutralizing Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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