Mouse Anti-EGFRvIII Recombinant Antibody (clone D2C7) (CAT#: HPAB-0111LY)
This product is antibody recognizes EGFRvIII. The antibody D2C7 can be used in some immunoassay techniques such as: FC, In vitro cytotoxicity assay.
Specific Inquiry
ELISA
Figure 1 Determination of the epitope of D2C7 on EGFRvIII ECD.
The wells were blocked with superblock and incubated with D2C7 mAb followed by peroxidase conjugated anti-mouse IgG antibody. Following the addition of TMB substrate solution, and stop solution, the optical density was measured at 450 nm. The ELISA assay with different concentrations of Nus-Tag alone or Nus-Tag conjugated to EGFRvIII ECD deletion mutants was conducted in triplicate for each epitope.
Chandramohan, V., Bao, X., Keir, S. T., Pegram, C. N., Szafranski, S. E., Piao, H., ... & Bigner, D. D. (2013). Construction of an immunotoxin, D2C7-(scdsFv)-PE38KDEL, targeting EGFRwt and EGFRvIII for brain tumor therapy. Clinical Cancer Research, 19(17), 4717-4727.
FC
Figure 2 Flow cytometric analysis of D2C7-(scdsFv)-PE38KDEL to determine reactivity of the D2C7 immunotoxin.
Parental NR6 cells were used as control. Indirect FACS analysis shows the reactivity of D2C7- (scdsFv)-PE38KDEL immunotoxin with cells expressing EGFRwt (NR6W).
Chandramohan, V., Bao, X., Keir, S. T., Pegram, C. N., Szafranski, S. E., Piao, H., ... & Bigner, D. D. (2013). Construction of an immunotoxin, D2C7-(scdsFv)-PE38KDEL, targeting EGFRwt and EGFRvIII for brain tumor therapy. Clinical Cancer Research, 19(17), 4717-4727.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human EGFRvIII
- Species Reactivity
- Human
- Clone
- D2C7
- Applications
- FC, Cyt
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The EGFRvIII antibody has been reported in applications of Flow Cytometry, Cytotoxicity, Enzyme-linked Immunosorbent Assay.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone D2C7"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-310 | Recombinant Anti-EGFRvIII Antibody | ELISA, WB, Dot, FuncS | IgG |
| MHH-310 | Recombinant Human Anti-EGFRvIII Antibody | IF, ELISA, FuncS | IgG |
| HPAB-0483LY | Human Anti-EGFRvIII Recombinant Antibody (clone CBL009LY) | ELISA | Human IgG |
| HPAB-648-FY | Human Anti-EGFRvIII Recombinant Antibody (HPAB-648-FY) | FC, ELISA | Human IgG |
| HPAB-M0065-YC | Human Anti-EGFRvIII Recombinant Antibody (HPAB-M0065-YC) | FC, Block, FuncS | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-310-F(E) | Recombinant Anti-EGFRvIII Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| MHH-310-F(E) | Recombinant Human Anti-EGFRvIII Antibody Fab Fragment | RIA, ELISA, FuncS | Fab |
| HPAB-0111LY-F(E) | Mouse Anti-EGFRvIII Recombinant Antibody (clone D2C7); Fab Fragment | FC, Cyt | Mouse Fab |
| HPAB-0483LY-F(E) | Human Anti-EGFRvIII Recombinant Antibody (clone CBL009LY); Fab Fragment | ELISA | Human Fab |
| HPAB-M0270-YC-F(E) | Human Anti-EGFRvIII Recombinant Antibody; Fab Fragment (HPAB-M0270-YC-F(E)) | FC | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0111LY-S(P) | Mouse Anti-EGFRvIII Recombinant Antibody (clone D2C7); scFv Fragment | FC, Cyt | Mouse scFv |
| HPAB-M0065-YC-S(P) | Human Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-M0065-YC-S(P)) | FC, Block, FuncS | Human scFv |
| HPAB-M0066-YC-S(P) | Mouse Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-M0066-YC-S(P)) | FC | Mouse scFv |
| HPAB-M0067-YC-S(P) | Human Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-M0067-YC-S(P)) | FC | Human scFv |
| HPAB-M0270-YC-S(P) | Human Anti-EGFRvIII Recombinant Antibody; scFv Fragment (HPAB-M0270-YC-S(P)) | FC | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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