Mouse Anti-EIF4E Recombinant Antibody (VS3-CJ444) (CAT#: VS3-CJ444)
This product is a mouse antibody that recognizes human, mouse, and rat EIF4E.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68358/1607_H9_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68358/1607_H9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16316/951_E2_2_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16316/951_E2_2_selected.jpg
Figure 3 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_6_6.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4077/11592_A_8_5.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151247-EIF4E
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000151247-EIF4E
Specifications
- Immunogen
- Recombinant protein of human IF4E
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat EIF4E
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:4000), Immunocytochemistry (1:25).
Target
- Alternative Names
- CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
- Gene ID
- 1977
- UniProt ID
- P06730
- Sequence Similarities
- Belongs to the eukaryotic initiation factor 4E family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
- Protein Refseq
- NP_001124150.1; NP_001124151.1; NP_001317946.1
- Function
- Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity).
In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285).
May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Related Resources
Insulin Signaling Pathway
MAPK Signaling Pathway
mTOR Signaling Pathway
Insulin Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "EIF4E"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1128 | Hi-Affi™ Rabbit Anti-EIF4E Recombinant Antibody (clone DS1128AB) | WB, IP, IHC-P, ICC | Rabbit IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1838-CN | Rabbit Anti-EIF4E Polyclonal Antibody (MRO-1838-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0648J | Rabbit Anti-EIF4E Recombinant Antibody (clone 1F3) | ELISA, WB, IHC | Rabbit IgG |
| VS3-QX360 | Mouse Anti-EIF4E Recombinant Antibody (clone 8C8-C10-C12) | WB | Mouse IgG1 |
| VS3-QX361 | Mouse Anti-EIF4E Recombinant Antibody (clone 163CT48.1.9) | WB, ICC | Mouse IgG1, κ |
| VS3-FY489 | Recombinant Rabbit Anti-EIF4E Antibody (clone R04-4H7) | WB, IHC-P, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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