Rabbit Anti-EIF5A Recombinant Antibody (VS3-CJ1103) (CAT#: VS3-CJ1103)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat EIF5A

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IHC, IP, FC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.

• Alternative Names
• FABAS; EIF-5A; EIF5A1; eIF-4D; Eif5ai

• Gene ID
• 1984

• UniProt ID
• P63241

• Sequence Similarities
• Belongs to the eIF-5A family.

• Cellular Localization
• Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

• Post Translation Modifications
• Acetylated by PCAF/KAT2B, regulating its subcellular localization (PubMed:19379712, PubMed:22771473). Deacetylated by SIRT2 (PubMed:22771473).
  Lys-50 undergoes hypusination, a unique post-translational modification that consists in the addition of a butylamino group from spermidine to lysine side chain, leading to the formation of the unusual amino acid hypusine. eIF-5As are the only known proteins to undergo this modification, which is essential for their function.

• Protein Refseq
• NP_001137232.1; NP_001137233.1; NP_001137234.1

• Function
• mRNA-binding protein involved in translation elongation (PubMed:16987817, PubMed:17187778, PubMed:33547280).
  Has an important function at the level of mRNA turnover, probably acting downstream of decapping (PubMed:16987817, PubMed:17187778, PubMed:33547280).
  Critical for the efficient synthesis of peptide bonds between consecutive proline residues (PubMed:16987817, PubMed:17187778, PubMed:33547280).
  Can resolve ribosomal stalling caused by consecutive prolines during translation (PubMed:16987817, PubMed:17187778, PubMed:33547280).
  Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed:16987817, PubMed:17187778, PubMed:33547280).
  With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed:15371445).
  Regulates also TNF-alpha-mediated apoptosis (PubMed:15452064).
  Mediates effects of polyamines on neuronal process extension and survival (PubMed:17360499).
  May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
  Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts (PubMed:8253832).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody