This product is a mouse antibody that recognizes ENO2.
Figure 1 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IF
Immunofluorescence analysis of Hela cells. 1. Cdk2 polyclonal antibody (red) was diluted at 1:200 (overnight at 4°C). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (overnight at 4°C). 2. Goat anti-rabbit Alexa Fluor 594 diluted 1:1000 (room temperature, 50 minutes). Goat Anti Mouse Alexa Fluor 488 is diluted 1:1000 (room temperature, 50 minutes).
Figure 2 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
Figure 3 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IHC
Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1. The antibody is diluted 1:200 (overnight at 4°C). 2. Sodium citrate pH 6.0 is used for antibody repair (>98°C, 20 minutes). 3. Dilute the secondary antibody at 1:200 (room temperature, 30 minutes). The negative control is only used by the secondary antibody.
Figure 4 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in IF
Immunofluorescence analysis of human appendix tissue. 1. NSE monoclonal antibody (13E2) (red) is diluted at 1:200 (4°C, overnight). 2. The Cy3 labeled secondary antibody was diluted 1:300 (room temperature, 50 minutes). 3. Picture B: DAPI (blue) for 10 minutes. Figure A: Goal. Picture B: DAPI. Picture C: The merger of A+B.
Figure 5 Mouse Anti-ENO2 Recombinant Antibody (clone 13E2) (MOB-0145F) in WB
1) Western blot analysis of Hela, 2) Jurkat, 3) 293T cell lysates, diluted 1:3000.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0694MZ | Chicken Anti-ENO2 Polyclonal IgY | ICC/IF, IHC, WB | Chicken antibody |
BRD-0817MZ | Chicken Anti-NSE Polyclonal IgY | IF, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1152 | Hi-Affi™ Rabbit Anti-ENO2 Recombinant Antibody (clone DS1152AB) | WB, FC, IHC-P, ICC | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-1114-CN | Rabbit Anti-ENO2 Recombinant Antibody (clone CBACN-409) | WB, IF, IHC | Rabbit IgG |
VS3-WK762 | Mouse Anti-ENO2 Recombinant Antibody (clone NS001) | WB, ELISA | Mouse IgG |
VS3-WK763 | Mouse Anti-ENO2 Recombinant Antibody (clone NS002) | WB, ELISA | Mouse IgG |
VS3-WK764 | Mouse Anti-ENO2 Recombinant Antibody (clone NS003) | WB, ELISA | Mouse IgG |
VS3-WK765 | Mouse Anti-ENO2 Recombinant Antibody (clone NS005) | ELISA, CLIA, RIA, IHC, WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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