Human Anti-EPHA2 Recombinant Antibody (clone 3F2) (CAT#: HPAB-0167CQ)

This product is a recombinant human antibody that can bind to human EPHA2 with high affinity.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
FuncS

Figure 1 The nucleotide and deduced amino acid sequence of the variable region of the anti-EphA2 antibody 3F2.

Figure 1 The nucleotide and deduced amino acid sequence of the variable region of the anti-EphA2 antibody 3F2.

ELISA

Figure 2 ELISA analysis of the wild type anti-EphA2 anti body 3F2 and the 3F2 I332E (3F2-1M) and 3F2 S239D/A330L/I332E (3F2-3M) Fc variants binding to FcγRIIIA tetramer.

Figure 2 ELISA analysis of the wild type anti-EphA2 anti body 3F2 and the 3F2 I332E (3F2-1M) and 3F2 S239D/A330L/I332E (3F2-3M) Fc variants binding to FcγRIIIA tetramer.

Both the 3F2-1M and 3F2-3M Fc variants bind better to FcγRIIIA tetramers.

FC

Figure 3 FACS analysis of anti-EphA2 antibody 3F2-WT, 3F2-1M and 3F2-3M binding to cells via Fc-domain interactions.

Figure 3 FACS analysis of anti-EphA2 antibody 3F2-WT, 3F2-1M and 3F2-3M binding to cells via Fc-domain interactions.

THP-1 and NK cells were stained with antibodies to FcγRI, FcγRII and FcγRIII.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human EPHA2
  • Species Reactivity
  • Human
  • Clone
  • 3F2
  • Applications
  • ELISA, IF, FC, FuncS
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry and Functional Assay. Please see the Published Data for further information.

Target

  • Alternative Names
  • EPH Receptor A2; Tyrosine-Protein Kinase Receptor ECK; EC 2.7.10.1; ECK; Epithelial Cell Receptor Protein Tyrosine Kinase; Ephrin Type-A Receptor 2; Soluble EPHA2 Variant 1; Epithelial Cell Kinase;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 3F2"

See other products for "EPHA2"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1164 Hi-Affi™ Rabbit Anti-EPHA2 Recombinant Antibody (clone DS1164AB) WB Rabbit IgG

Recombinant Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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