Human Anti-Estradiol Recombinant Antibody (clone Est) (CAT#: FAMAB-0026JF)

Recombinant monoclonal antibody is capable of specifically reacting with human estradiol. This human anti-estradiol antibody (Est) is important in the regulation of the estrous and menstrual female reproductive cycles.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Datasheet
  • MSDS
  • COA
FuncS

Figure 1 Standard curve for estradiol assay.

Figure 1 Standard curve for estradiol assay.

The competitive immunoassay was performed with the mutant 4aa16/N50Y+F83S.

Dhar, T. K., Samanta, A. K., & Ali, E. (1988). Homogeneous enzyme immunoassay of estradiol using estradiol-3-0-carboxymethyl ether as hapten. Steroids, 51(5-6), 519-526.

ELISA

Figure 2 Standard curve for estimation of estradiol using estradiol mAb.

Figure 2 Standard curve for estimation of estradiol using estradiol mAb.

Antibody dilution 1:10.

Dhar, T. K., Samanta, A. K., & Ali, E. (1988). Homogeneous enzyme immunoassay of estradiol using estradiol-3-0-carboxymethyl ether as hapten. Steroids, 51(5-6), 519-526.

IHC

Figure 3 lmmunohistochemical staining for D5 in case 5.

Figure 3 lmmunohistochemical staining for D5 in case 5.

Specific staining for ER is located principally in the cytoplasm of cancer cells.

Inoue, H., Oshimo, K., Miki, H., Kawano, M., Komaki, K., Monden, Y., ... & Tsuyuguchi, M. (1993). Immunohistochemical study of estrogen receptor and estradiol on papillary thyroid carcinoma in young patients. Journal of surgical oncology, 53(4), 226-230.


Specifications

  • Host Species
  • Human
  • Derivation
  • Human phage display scFv library
  • Type
  • Human IgG
  • Specificity
  • Human estradiol
  • Species Reactivity
  • Human
  • Clone
  • Est
  • Applications
  • ELISA, FuncS, IHC
  • Related Disease
  • Neutralising overdoses

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Estradiol antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Functional Assay, Immunohistochemistry.

Target

  • Alternative Names
  • Estradiol

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone Est"

See other products for "Estradiol"

Mouse Antibody

Human Antibody

CAT Product Name Application Type
PABX-055-S (P) Recombinant Mouse Anti-Estradiol Antibody scFv Fragment (57-2) WB, ELISA, FuncS scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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