Mouse Anti-FAP Recombinant Antibody (clone F19) (CAT#: HPAB-A1368-YJ)

Figure 1 Immunoprecipitation of purified FAPmCD8 fusion protein followed by 8% SDS-polyacrylamide gel electrophoresis and dipeptidyl peptidase overlay assay.

Park, J. E., Lenter, M. C., Zimmermann, R. N., Garin-Chesa, P., Old, L. J., & Rettig, W. J. (1999). Fibroblast activation protein, a dual specificity serine protease expressed in reactive human tumor stromal fibroblasts. Journal of Biological Chemistry, 274(51), 36505-36512.

Figure 2 Immunofluorescence microscopy of 293FAPwt transfectants (A) and vector-transfected parental 293 cells (B) with antiFAP mAb F19 followed by fluorescein isothiocyanate-labeled goat antimouse IgG.

Figure 3 Immunohistochemical detection of FAP using mAb F19 in a colon carcinoma (A) or matched control normal colon tissue from the same patient (B).

Figure 4 FAP activity was measured using concanavalin A-enriched membrane fractions, followed by binding to F19-coated plates and incubation with Ala-Pro-AFC as substrate.

Figure 5 Epitope analysis of FAP-specific MAbs.

Figure 6 Analysis of FAPa cell surface expression on FB20 leptomeningeal fibroblasts treated with growth and differentiation factors for 24 hr.

Indirect radiobinding assays were carried out with MAb F19 or negative control mouse IgG, followed by lEI-goat anti-mouse Ig.

Rettig, W. J., Su, S. L., Fortunato, S. R., Scanlan, M. J., Mohan Raj, B. K., Garin‐Chesa, P., ... & Old, L. J. (1994). Fibroblast activation protein: purification, epitope mapping and induction by growth factors. International journal of cancer, 58(3), 385-392.

Figure 1 Anti-FAP Antibody in WB.

Western blot analysis of HPAB-A1368-YJ was performed by loading Human FAP Protein.

Figure 2 Anti-FAP Antibody in ELISA.

ELISA analysis of HPAB-A1368-YJ was performed by coating with Human FAP Protein.