Mouse Anti-FLT4 Recombinant Antibody (VS3-CJ913) (CAT#: VS3-CJ913)

• Immunogen
• Synthesized peptide derived from human VEGFR3

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human FLT4

• Species Reactivity
• Human

• Applications
• IHC

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Immunohistochemistry (1:200-1:500).

• Alternative Names
• PCL; CHTD7; FLT-4; FLT41; LMPH1A; LMPHM1; VEGFR3; VEGFR-3

• Gene ID
• 2324

• UniProt ID
• P35916

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

• Cellular Localization
• Cell membrane, Cytoplasm, Membrane, Nucleus, Secreted

• Post Translation Modifications
• Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation in response to H2O2 is mediated by a process that requires SRC and PRKCD activity. Phosphorylation at Tyr-1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at Tyr-1063 and Tyr-1337 is important for interaction with CRK and subsequent activation of MAPK8. Phosphorylation at Tyr-1230, Tyr-1231 and Tyr-1337 is important for interaction with GRB2 and subsequent activation of the AKT1 and MAPK1/ERK2 and/or MAPK3/ERK1 signaling pathways. In response to endothelial cell adhesion onto collagen, can also be phosphorylated in the absence of FLT4 kinase activity by SRC at Tyr-830, Tyr-833, Tyr-853, Tyr-1063, Tyr-1333, and Tyr-1337.

• Protein Refseq
• NP_002011.2; NP_891555.2

• Function
• Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS