The antibody clone Fn52 was produced by immunizing with purified human Fibronectin. It can be used for detection of human Fibronectin in ELISA, WB and IF staining.
Figure 1 Western blot images of full length fibronectin with scFv Fn52.
Full length fibronectin using anti-fibronectin antibody (lane 1); scFv Fn52 as primary antibody for full length fibronectin (lane 2), 70 kDa fragment of fibronectin(lane 3) and the 30 kDa fragment of fibronectin (lane 4) respectively.
Figure 2 Fn52 and Fn52RGDS inhibit the migration of D407 RPE cells.
RPE cells were pre-incubated for 1 h with the various reagents, at indicated concentrations, and added to the upper chamber of the transwell insert, while the bottom chamber in each well, contained Fn at a concentration of 20 mg/ml (control).
Figure 3 Fn52 and Fn52RGDS inhibit the fibronectin polymerization.
Immunocytochemistry of ARPE-19 retinal pigment epithelial cells, cultured in the presence of patient-derived vitreous and subretinal fluid, in the presence and absence of scFv antibodies.
Figure 4 Fn52 and Fn52RGDS inhibit the MMP expression in ARPE-19 RPE cells.
lane 1: no scFv; lane 2: scFvO27; lane 3: scFv Fn52; lane 4: scFv Fn52RGDS.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-193 | Anti-Human Fibronectin Extra Domain-B Recombinant Antibody (Radretumab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | [(scFv - heavy - kappa) - IGHE - CH4]2 |
TAB-001CQ | Anti-Human FN1 Recombinant Antibody (MOR03255) | ELISA, Block, IHC | Human antibody |
TAB-002CQ | Anti-Human FN1 Recombinant Antibody (ME-4C) | ELISA, IHC | Human antibody |
TAB-071WM | Anti-Human FN1 Recombinant Antibody (CGS-1) | ELISA, WB | Human Antibody |
TAB-071WM-F(E) | Anti-Human FN1 Recombinant Antibody Fab Fragment (CGS-1) | ELISA, WB | Human Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-0025MZ | Recombinant Mouse Anti-Human Fibronectin Antibody (clone C715M) | ELISA | Mouse antibody |
HPAB-0193-CN | Mouse Anti-FN1 Recombinant Antibody (HPAB-0193-CN) | ELISA, FC, IHC | Mouse IgG1 |
HPAB-1705-FY | Mouse Anti-FN1 Recombinant Antibody (clone MOR02610) | ELISA, IHC | Mouse IgG |
HPAB-1706-FY | Mouse Anti-FN1 Recombinant Antibody (clone MOR02611) | ELISA, IHC | Mouse IgG |
HPAB-1707-FY | Mouse Anti-FN1 Recombinant Antibody (clone MOR02613) | ELISA, IHC | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-852CQ | Mouse Anti-FN1 Recombinant Antibody (clone HFN7.1) | ELISA, WB, IP, Block | Mouse IgG1 |
NEUT-853CQ | Mouse Anti-FN1 Recombinant Antibody (clone IST-9) | WB, RIA, ELISA, IHC-Fr, ICC, IF, Block, IHC-P | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1326 | Hi-Affi™ Rabbit Anti-FN1 Recombinant Antibody (clone DS1326AB) | IHC-Fr, IHC-P, IHC-P, IP, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0193-CN-S(P) | Mouse Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0193-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
HPAB-0194-CN-S(P) | Mouse Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-0194-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
FAMAB-0105-YC-S(P) | Human Anti-FN1 Recombinant Antibody (clone ME4C); scFv Fragment | ELISA, IHC | Human scFv |
HPAB-S0037-YC-S(P) | Human Anti-FN1 Recombinant Antibody (clone CGS-2); scFv Fragment | ELISA, IB, IHC | Human scFv |
HPAB-S0038-YC-S(P) | Human Anti-FN1 Recombinant Antibody (clone L19); scFv Fragment | ELISA, IF, IHC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0193-CN-F(E) | Mouse Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0193-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
HPAB-0194-CN-F(E) | Mouse Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0194-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
HPAB-0195-CN-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0195-CN-F(E)) | ELISA, FC, IHC | Humanized Fab |
HPAB-0196-CN-F(E) | Human Anti-FN1 Recombinant Antibody; Fab Fragment (HPAB-0196-CN-F(E)) | ELISA, FC, IHC | Humanized Fab |
HPAB-1110-FY-S(P) | Human Anti-FN1 Recombinant Antibody; scFv Fragment (HPAB-1110-FY-S(P)) | IHC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
NBD-0040-YJ-VHH | Camelid Anti-FN1 Recombinant Single Domain Antibody (NBD-0040-YJ-VHH) | ELISA, In vivo imaging | Camelid VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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