Mouse Anti-FN1 Recombinant Antibody (clone M9) (CAT#: ZG-0035C)

This product is a mouse antibody that recognizes FN1. It can be used in a wide variety of applications, such as: WB, IHC-P, IF/ICC.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IHC

Figure 1 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 1 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Fibronectin Monoclonal Antibody (M9) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IHC

Figure 2 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 2 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Fibronectin Monoclonal Antibody (M9) was diluted at 1:200 (4° C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval (>98° C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min). Negative control was used by Secondary antibody only.

IF

Figure 3 Mouse Anti-FN1 Antibody (clone M9) in IF

Figure 3 Mouse Anti-FN1 Antibody (clone M9) in IF

Immunofluorescence analysis of Human-appendix tissue. 1, Fibronectin Monoclonal Antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

IF

Figure 4 Mouse Anti-FN1 Antibody (clone M9) in IF

Figure 4 Mouse Anti-FN1 Antibody (clone M9) in IF

Immunofluorescence analysis of Mouse-spleen tissue. 1, Fibronectin Monoclonal Antibody (M9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50 min). 3, Picture B: DAPI (blue) 10 min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

WB

Figure 5 Mouse Anti-FN1 Antibody (clone M9) in WB

Figure 5 Mouse Anti-FN1 Antibody (clone M9) in WB

WB analysis of Hela, diluted at 1:2000.

IHC

Figure 6 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 6 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).

IHC

Figure 7 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 7 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).

IHC

Figure 8 Mouse Anti-FN1 Antibody (clone M9) in IHC

Figure 8 Mouse Anti-FN1 Antibody (clone M9) in IHC

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4° overnight). 2, High-pressure and temperature EDTA, pH 8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30 min).


Specifications

  • Immunogen
  • Synthetic Peptide of Fibronectin
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Fibronectin
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • M9
  • Applications
  • WB, IHC-P, IF/ICC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Storage
  • Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence. For details, refer to Tested Data.

Target

  • Alternative Names
  • Fibronectin 1; Cold-Insoluble Globulin; Migration-Stimulating Factor; CIG; FN; Fibronectin; GFND2; SMDCF

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "FN1"

Blocking Antibody

CAT Product Name Application Type
NEUT-852CQ Mouse Anti-FN1 Recombinant Antibody (clone HFN7.1) ELISA, WB, IP, Block Mouse IgG1
NEUT-853CQ Mouse Anti-FN1 Recombinant Antibody (clone IST-9) WB, RIA, ELISA, IHC-Fr, ICC, IF, Block, IHC-P Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1326 Hi-Affi™ Rabbit Anti-FN1 Recombinant Antibody (clone DS1326AB) IHC-Fr, IHC-P, IHC-P, IP, WB Rabbit IgG

Single-domain Antibody

CAT Product Name Application Type
NBD-0040-YJ-VHH Camelid Anti-FN1 Recombinant Single Domain Antibody (NJB2) ELISA, In vivo imaging Camelid VHH

Recombinant Antibody

CAT Product Name Application Type
ZG-0661F Rabbit Anti-FN1 Recombinant Antibody (ZG-0661F) IF, IHC-P, ELISA, RIA Rabbit IgG
ZG-0662F Rabbit Anti-FN1 Recombinant Antibody (ZG-0662F) IF, IHC-P, ELISA, RIA Rabbit IgG
ZG-0663F Rabbit Anti-FN1 Recombinant Antibody (ZG-0663F) IF, IHC-P, ELISA, RIA Rabbit IgG
ZG-0664F Rabbit Anti-FN1 Recombinant Antibody (ZG-0664F) IHC-P, IF, ELISA, RIA, WB Rabbit IgG
ZG-0665F Rabbit Anti-FN1 Recombinant Antibody (ZG-0665F) IHC-P, IF, ELISA, RIA, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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