Rabbit Anti-FOXC1 Recombinant Antibody (clone R06-3B5) (CAT#: VS3-FY1722)

• Immunogen
• Recombinant human FOXC1 protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Mouse FOXC1

• Species Reactivity
• Human, Mouse

• Clone
• R06-3B5

• Applications
• Western Blot, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 57 kDa, while the observed molecular weight stands at 75 kDa.

• Related Disease
• Anterior Segment Dysgenesis 3 and Axenfeld-Rieger Syndrome, Type 3

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• Forkhead Box C1; Forkhead-Related Transcription Factor 3; Forkhead-Related Protein FKHL7; FREAC-3; FREAC3; FKHL7; Forkhead/Winged Helix-Like Transcription Factor 7; Forkhead, Drosophila, Homolog-Like 7; Forkhead-Related Activator 3; Forkhead Box C1 Protein

• Gene ID
• 2296

• UniProt ID
• Q12948

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated (PubMed:11782474, PubMed:19279310, PubMed:25786029).
  Phosphorylated on Ser-272 in response to epidermal growth factor (EGF) in a ERK1/2 MAPK-dependent signaling pathway; phosphorylation contributes to its protein stability and transcriptional activity (PubMed:16492674).

• Protein Refseq
• NP_001444.2

• Function
• Acts as a transcriptional coactivator.
  Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification (By similarity).
  Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells.
  Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody